Synthesis and Properties of PGS-Li Scaffold

doi:10.1007/s40242-018-8164-4

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (4): 670-675.doi: 10.1007/s40242-018-8164-4

Synthesis and Properties of PGS-Li Scaffold

LIU Zhihui¹, LU Yadong¹, FENG Wei³, YANG Junxing¹, GAO Shang¹, SONG Lijie¹, WANG Yao¹, WANG Bowei²

1. Stomatology Hospital of Jilin University, Changchun 130012, P. R. China;
2. The Second Hospital of Jilin University, Changchun 130022, P. R. China;
3. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China

收稿日期:2018-05-17 出版日期:2018-08-01 发布日期:2018-06-19
通讯作者: WANG Bowei E-mail:wangbowei2009@hotmail.com
基金资助:
Supported by the Development Project of the Pharmaceutical Industry of Jilin Province, China(Nos.201603028YY, 20150311070YY), the "Double Ten Project" of Changchun City, China(No.16SS12), the Key Science and Technology Project of Jilin Province, China(No.20150204004YY) and the Graduate Innovation Fund of Jilin University, China(No.2017134).

Synthesis and Properties of PGS-Li Scaffold

LIU Zhihui¹, LU Yadong¹, FENG Wei³, YANG Junxing¹, GAO Shang¹, SONG Lijie¹, WANG Yao¹, WANG Bowei²

1. Stomatology Hospital of Jilin University, Changchun 130012, P. R. China;
2. The Second Hospital of Jilin University, Changchun 130022, P. R. China;
3. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China

Received:2018-05-17 Online:2018-08-01 Published:2018-06-19
Contact: WANG Bowei E-mail:wangbowei2009@hotmail.com
Supported by:
Supported by the Development Project of the Pharmaceutical Industry of Jilin Province, China(Nos.201603028YY, 20150311070YY), the "Double Ten Project" of Changchun City, China(No.16SS12), the Key Science and Technology Project of Jilin Province, China(No.20150204004YY) and the Graduate Innovation Fund of Jilin University, China(No.2017134).

摘要/Abstract

摘要： Lithium ion-doped polyglycerol sebacate scaffold(PGS)-Li was synthesized by adding lithium ions to polyglycerol sebacate(PGS) during its crosslinking process due to the specific effects of lithium ions on periodontal ligament cells, cementoblasts and the eminent performance of PGS. The molecular mass, composition, structure, porosity, thermal properties, and hydrophilicity of the composite were characterized by gel permeation chromatography(GPC), Fourier transform infrared spectroscopy(FTIR), inductively coupled plasma optical emission spectrometer(ICP-OES), scanning electron microscopy(SEM), X-ray photoelectron spectroscopy(XPS), thermogravimetric analyzer(TGA) and contact angle measurments, and the degradation of the material was evaluated by in vitro degradation experiments. The biological activity of PGS-Li scaffold was detected by calcein-AM staining and cytotoxicity test. The results indicate that PGS-Li scaffold has been successfully synthesized, which has similar composition and structure to PGS, but slightly larger molecular weight. In addition, the porosity and pore size of PGS-Li scaffold ba-sically meet the requirements of engineering scaffold materials and the seaffold shows better performance in terms of hydrophilicity and thermal stability than PGS. In vitro degradation experimental results show that the degradation rate of PGS-Li scaffold is higher than that of PGS. What's more, the results of cytotoxicity test and cell staining show that there is no significant difference in the proliferation and cell morphology of cementoblasts.

关键词: Lithium, Polyglycerol sebacate(PGS), Tissue engineering, Dental cementum

Abstract: Lithium ion-doped polyglycerol sebacate scaffold(PGS)-Li was synthesized by adding lithium ions to polyglycerol sebacate(PGS) during its crosslinking process due to the specific effects of lithium ions on periodontal ligament cells, cementoblasts and the eminent performance of PGS. The molecular mass, composition, structure, porosity, thermal properties, and hydrophilicity of the composite were characterized by gel permeation chromatography(GPC), Fourier transform infrared spectroscopy(FTIR), inductively coupled plasma optical emission spectrometer(ICP-OES), scanning electron microscopy(SEM), X-ray photoelectron spectroscopy(XPS), thermogravimetric analyzer(TGA) and contact angle measurments, and the degradation of the material was evaluated by in vitro degradation experiments. The biological activity of PGS-Li scaffold was detected by calcein-AM staining and cytotoxicity test. The results indicate that PGS-Li scaffold has been successfully synthesized, which has similar composition and structure to PGS, but slightly larger molecular weight. In addition, the porosity and pore size of PGS-Li scaffold ba-sically meet the requirements of engineering scaffold materials and the seaffold shows better performance in terms of hydrophilicity and thermal stability than PGS. In vitro degradation experimental results show that the degradation rate of PGS-Li scaffold is higher than that of PGS. What's more, the results of cytotoxicity test and cell staining show that there is no significant difference in the proliferation and cell morphology of cementoblasts.

Key words: Lithium, Polyglycerol sebacate(PGS), Tissue engineering, Dental cementum

LIU Zhihui, LU Yadong, FENG Wei, YANG Junxing, GAO Shang, SONG Lijie, WANG Yao, WANG Bowei. Synthesis and Properties of PGS-Li Scaffold[J]. 高等学校化学研究, 2018, 34(4): 670-675.

LIU Zhihui, LU Yadong, FENG Wei, YANG Junxing, GAO Shang, SONG Lijie, WANG Yao, WANG Bowei. Synthesis and Properties of PGS-Li Scaffold[J]. Chemical Research in Chinese Universities, 2018, 34(4): 670-675.

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Synthesis and Properties of PGS-Li Scaffold

Synthesis and Properties of PGS-Li Scaffold

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