Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (4): 541-545.

Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

ZHANG Jin-lei^1,2, TAN Xue-cai^1*, ZHAO Dan-dan¹, TAN Sheng-wei¹, HUANG Zeng-wei¹, MI Yan¹ and HUANG Zai-yin¹

1. College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, P. R. China;
2. Department of Chemistry and Life Science, Baise University, Baise 533000, P. R. China

收稿日期:2009-09-16 修回日期:2009-11-16 出版日期:2010-07-25 发布日期:2010-10-01
通讯作者: TAN Xue-cai
基金资助:
Supported by the Natural Science Foundation of Guangxi Province, China(Nos.0639025 and 0991084), the Support Program for 100 Young and Middle-aged Disciplinary Leaders in Higher Education Institutions of Guangxi Province, China(No. RC20060703005), the Key Laboratory of Development and Application of Forest Chemicals of Guangxi Province, China(No.GXFC08-06), the Education Department of Guangxi Province, China(No.200807MS074) and the Innovation Project of Guangxi University for Nationalities, China(No.gxun-chx2009081).

Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

ZHANG Jin-lei^1,2, TAN Xue-cai^1*, ZHAO Dan-dan¹, TAN Sheng-wei¹, HUANG Zeng-wei¹, MI Yan¹ and HUANG Zai-yin¹

1. College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, P. R. China;
2. Department of Chemistry and Life Science, Baise University, Baise 533000, P. R. China

Received:2009-09-16 Revised:2009-11-16 Online:2010-07-25 Published:2010-10-01
Contact: TAN Xue-cai
Supported by:
Supported by the Natural Science Foundation of Guangxi Province, China(Nos.0639025 and 0991084), the Support Program for 100 Young and Middle-aged Disciplinary Leaders in Higher Education Institutions of Guangxi Province, China(No. RC20060703005), the Key Laboratory of Development and Application of Forest Chemicals of Guangxi Province, China(No.GXFC08-06), the Education Department of Guangxi Province, China(No.200807MS074) and the Innovation Project of Guangxi University for Nationalities, China(No.gxun-chx2009081).

摘要/Abstract

摘要： A novel third-generation hydrogen peroxide(H₂O₂) biosensor(Hb/CdS/MWNTs/GCE) was fabricated through hemoglobin(Hb) adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes’(MWNTs) films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential(E⁰') of –0.230 V(vs. Ag/AgCl) in 0.1 mol/L pH=8.0 phosphate buffer solution(PBS), which was the characteristic of the Hb heme Fe(III)/Fe(II) redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H₂O₂. The response time of the designed biosensor to H₂O₂ at a potential of –0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^–6―2.7×10^–3 mol/L and 2.7×10^–3―7.7×10^–3 mol/L with a detection limit of 3.0×10^–7 mol/L(S/N=3). The apparent Michaelis-Menten constant K_m was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.

关键词: Hemoglobin, CdS quantum dot, Multi-wall carbon nanotube, Biosensor, Direct electrochemistry

Abstract: A novel third-generation hydrogen peroxide(H₂O₂) biosensor(Hb/CdS/MWNTs/GCE) was fabricated through hemoglobin(Hb) adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes’(MWNTs) films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential(E⁰') of –0.230 V(vs. Ag/AgCl) in 0.1 mol/L pH=8.0 phosphate buffer solution(PBS), which was the characteristic of the Hb heme Fe(III)/Fe(II) redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H₂O₂. The response time of the designed biosensor to H₂O₂ at a potential of –0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^–6―2.7×10^–3 mol/L and 2.7×10^–3―7.7×10^–3 mol/L with a detection limit of 3.0×10^–7 mol/L(S/N=3). The apparent Michaelis-Menten constant K_m was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.

Key words: Hemoglobin, CdS quantum dot, Multi-wall carbon nanotube, Biosensor, Direct electrochemistry

ZHANG Jin-lei, TAN Xue-cai*, ZHAO Dan-dan, TAN Sheng-wei, HUANG Zeng-wei, .... Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots[J]. 高等学校化学研究, 2010, 26(4): 541-545.

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Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots

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