[an error occurred while processing this directive]

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (1): 70-74.

• Articles • 上一篇    下一篇

Differential Proteomic Analysis of Endometriosis

ZHANG Li-hui1, LIU Hong-yan1, LI He-lian1, ZHANG Xing-yi2 and SUN Mei3*   

  1. 1. Department of Gynecology & Obstetrics,
    2. Department of Thoracic Surgery,
    3. Department of Pathology, the Second Hospital of Jilin University, Changchun 130041, P. R. China
  • 收稿日期:2009-05-20 修回日期:2009-06-28 出版日期:2010-01-04 发布日期:2010-03-29
  • 通讯作者: SUN Mei, E-mail: sun.mei55@yahoo.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.30670301, 30870354).

Differential Proteomic Analysis of Endometriosis

ZHANG Li-hui1, LIU Hong-yan1, LI He-lian1, ZHANG Xing-yi2 and SUN Mei3*   

  1. 1. Department of Gynecology & Obstetrics,
    2. Department of Thoracic Surgery,
    3. Department of Pathology, the Second Hospital of Jilin University, Changchun 130041, P. R. China
  • Received:2009-05-20 Revised:2009-06-28 Online:2010-01-04 Published:2010-03-29
  • Contact: SUN Mei, E-mail: sun.mei55@yahoo.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30670301, 30870354).

PDF (PC)

摘要：

Differential proteins expressing in ectopic and eutopic endometria were investigated by means of proteomic analysis. Five patients in secretary phase were diagnosed as endometriosis by laparoscopy. The five ectopic endometria(two at stage II, two at stage III and one at stage IV) and five eutopic endometria were surgically excised. One-dimensional electrophoresis coupled with liquid chromatography and mass spectrometry was used to screen and identify differential proteins. Three differential bands in one-dimensional electrophoresis were resolved by liquid chromatography and mass spectrometry and 14 up-regulated proteins were identified, including collagen α-1, α-2, α-3(VI), α-1(XIV) chain, actin, annexin A2, EMILIN-1, ferritin light polypeptide variant, fucosyltransferase 10, myosin-9, protein S100-A9, KIAA1783 protein, and two hypothetical proteins. Our data provides a list of potential biomarkers for endometriosis. The identifications may be used to develop new diagnoses for endometriosis.

关键词: Endometriosis; One-dimensional electrophoresis; Liquid chromatography; Mass spectrometry; Proteomics

Abstract:

Differential proteins expressing in ectopic and eutopic endometria were investigated by means of proteomic analysis. Five patients in secretary phase were diagnosed as endometriosis by laparoscopy. The five ectopic endometria(two at stage II, two at stage III and one at stage IV) and five eutopic endometria were surgically excised. One-dimensional electrophoresis coupled with liquid chromatography and mass spectrometry was used to screen and identify differential proteins. Three differential bands in one-dimensional electrophoresis were resolved by liquid chromatography and mass spectrometry and 14 up-regulated proteins were identified, including collagen α-1, α-2, α-3(VI), α-1(XIV) chain, actin, annexin A2, EMILIN-1, ferritin light polypeptide variant, fucosyltransferase 10, myosin-9, protein S100-A9, KIAA1783 protein, and two hypothetical proteins. Our data provides a list of potential biomarkers for endometriosis. The identifications may be used to develop new diagnoses for endometriosis.

Key words: Endometriosis; One-dimensional electrophoresis; Liquid chromatography; Mass spectrometry; Proteomics