高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (2): 205-208.
A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems
XU Li1, LI Hong-mei2, HUANG Yi-bing1, WANG Hua3, NIE Guang-jun1, ZHANG Xue-zhong1
- 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, P.R.China;
2. College of Food Engineering, Jilin Agricultural University, Changchun 130018, P.R.China;
3. China-Japan Union Hospital of Jilin University, Changchun 130033, P.R.China
A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems
XU Li1, LI Hong-mei2, HUANG Yi-bing1, WANG Hua3, NIE Guang-jun1, ZHANG Xue-zhong1
- 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, P.R.China;
2. College of Food Engineering, Jilin Agricultural University, Changchun 130018, P.R.China;
3. China-Japan Union Hospital of Jilin University, Changchun 130033, P.R.China
摘要： Soybean bioactive peptides(SBPs) were prepared from the isolated soybean protein by proteolysis with an alkaline protease, alcalase, at 50 ℃ and pH = 8.0.The dependence of hydrolysis time on hydrolysis degree and molecular weight distribution were examined.The hydrolysate was fractionated on a Sephadex G-25 column and the anti-oxidative activities of the fractions were detected by the method of pyrogallol auto-oxidation.The average chain length of soybean peptides that have anti-oxidative activity was estimated to be about 7.The anti-oxidative properties of the soybean peptide were also studied by using linoleic acid peroxidation systems.The optimal condition of the peroxidation system was set up, Vc/Cu2+ as the inducer at pH = 7.4 and 25 ℃.In addition, soybean peptides show higher antioxidative activity compared with GSH.