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高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (2): 201-204.

• Articles • 上一篇    下一篇

Construction of High Expression Plasmid of Human Augmenter of Liver Regeneration(hALR), Expression and Purification of hALR

SUN Tian-xu, WU Yong-ge, YU Xiang-hui, JIANG Chun-lai, JIN Ying-hua, CHENG Yue, KONG Wei   

  1. Vaccine Research Center, College of Life Science, Jilin University, Changchun 130012, P.R.China
  • 收稿日期:2005-12-20 出版日期:2006-04-24 发布日期:2011-08-06

Construction of High Expression Plasmid of Human Augmenter of Liver Regeneration(hALR), Expression and Purification of hALR

SUN Tian-xu, WU Yong-ge, YU Xiang-hui, JIANG Chun-lai, JIN Ying-hua, CHENG Yue, KONG Wei   

  1. Vaccine Research Center, College of Life Science, Jilin University, Changchun 130012, P.R.China
  • Received:2005-12-20 Online:2006-04-24 Published:2011-08-06

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摘要： Experimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR)serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration.A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group.As a result, the protein was highly expressed in E.coli BL21.The recombinant hALR was over 60% of the total protein in E.coli.Its validity was confirmed by meansof Western Blotting.The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured.

关键词: Augmenter of liver regeneration, Prokayotic expression plasmid, FAD-dependent sulfhydryl oxidase

Abstract: Experimental evidence has been presented to suggest that the human augmenter of liver regeneration (hALR)serves as a hepatotrophic growth factor during liver regeneration and as a generalized growth factor during pancreas transplant/regeneration.A prokaryotic expression plasmid, pRSET/6his-c-myc-hALR was constructed, by cloning synthesized hALR cDNA into pRSET/6his-c-myc that was improved on the basis of pRSET B by the group.As a result, the protein was highly expressed in E.coli BL21.The recombinant hALR was over 60% of the total protein in E.coli.Its validity was confirmed by meansof Western Blotting.The protein was purified by Ni-NTA affinity chromatography and this FAD-dependent sulfhydryl oxidase activity was measured.

Key words: Augmenter of liver regeneration, Prokayotic expression plasmid, FAD-dependent sulfhydryl oxidase