Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

高等学校化学研究 ›› 1999, Vol. 15 ›› Issue (4): 333-336.

Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

GOU Xiao-jun¹, LI Xiang-hui¹, DING Tian-bing¹, WANG Yong-ting¹, CHEN Huai-yong¹, HUANG Cheng-fang¹, DONG Qing-chu¹, LI Qing-shan¹, WANG Yan-zhong², ZHAO Lu-lu³

1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
2. Medical Testing Depa rtment, People's Hospital of Jilin Province, Changchun 130021, P. R. China;
3. Changchun Higher College of Medical Sciences, Changchun 130031, P. R. China

收稿日期:1999-02-08 出版日期:1999-12-24 发布日期:2011-08-17
通讯作者: LI Qing-shan

Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

GOU Xiao-jun¹, LI Xiang-hui¹, DING Tian-bing¹, WANG Yong-ting¹, CHEN Huai-yong¹, HUANG Cheng-fang¹, DONG Qing-chu¹, LI Qing-shan¹, WANG Yan-zhong², ZHAO Lu-lu³

1. College of Life Science, Jilin University, Changchun 130023, P. R. China;
2. Medical Testing Depa rtment, People's Hospital of Jilin Province, Changchun 130021, P. R. China;
3. Changchun Higher College of Medical Sciences, Changchun 130031, P. R. China

Received:1999-02-08 Online:1999-12-24 Published:2011-08-17

摘要/Abstract

摘要： The Rana temporaria chensinensis is an amphibian distributing mainly over the north of China. Its skin gland contains a variety of peptides, including antibacterial peptides. One of them, component 1, has been purified from the dried skin of R.temporaria chensinesis using a five-step procedure. Purified component 1 has a molecular weight of 5700 and its amino acid composition shows that it is devoid of disulfide bridges but rich in histine and glycine residues. Moreover, it has been found that it has a broad spectrum of antibacterial activity for Pseuctomonas aeruginosa, Escherichia coli, Bacillus sublilis, S. faccalis and Proteus vulgaris .

关键词: Antibacterial activity, Peptide, Dried skin

Abstract: The Rana temporaria chensinensis is an amphibian distributing mainly over the north of China. Its skin gland contains a variety of peptides, including antibacterial peptides. One of them, component 1, has been purified from the dried skin of R.temporaria chensinesis using a five-step procedure. Purified component 1 has a molecular weight of 5700 and its amino acid composition shows that it is devoid of disulfide bridges but rich in histine and glycine residues. Moreover, it has been found that it has a broad spectrum of antibacterial activity for Pseuctomonas aeruginosa, Escherichia coli, Bacillus sublilis, S. faccalis and Proteus vulgaris .

Key words: Antibacterial activity, Peptide, Dried skin

GOU Xiao-jun, LI Xiang-hui, DING Tian-bing, WANG Yong-ting, CHEN Huai-yong, HUANG Cheng-fang, DONG Qing-chu, LI Qing-shan, WANG Yan-zhong, ZHAO Lu-lu . Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin[J]. 高等学校化学研究, 1999, 15(4): 333-336.

[1]	XU Min, ZHOU Baomei, DING Yan, DU Shanshan, SU Mengke, LIU Honglin. Programmable Oligonucleotide-Peptide Complexes:Synthesis and Applications[J]. 高等学校化学研究, 2022, 38(4): 856-865.
[2]	ZHANG Mengqian, HE Peiyang, LI Yanmei. Contemporary Approaches to α,β-Dehydroamino Acid Chemical Modifications[J]. 高等学校化学研究, 2021, 37(5): 1044-1054.
[3]	YANG Jia, ZHENG Rui, AN Hongwei, WANG Hao. In vivo Self-assembled Peptide Nanoprobes for Disease Diagnosis[J]. 高等学校化学研究, 2021, 37(4): 855-869.
[4]	LIU Zhiyu, LIANG Gaolin, ZHAN Wenjun. In situ Activatable Peptide-based Nanoprobes for Tumor Imaging[J]. 高等学校化学研究, 2021, 37(4): 889-899.
[5]	MENG Feihong, WANG Yajie, LU Tong, WANG Chunyu, LI Fei. Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP[J]. 高等学校化学研究, 2021, 37(3): 787-794.
[6]	ZHANG Ling, FU Yihong, DING Yue, MENG Jiao, WANG Zhenchao, WANG Peiyi. Antibacterial Activity of Novel 18β-Glycyrrhetinic Hydrazide or Amide Derivatives[J]. 高等学校化学研究, 2021, 37(3): 662-667.
[7]	WANG Lili, LIU Yanan, PENG Xiaoxing, SUN Yuanqing, LIU Xiaohua, LIU Huimin, LIN Quan, SUN Hongchen, YANG Bai, LI Xiangwei. Preparation and Characterization of CaO/ZnO Core-shell Structured Nanoparticles[J]. 高等学校化学研究, 2020, 36(5): 970-975.
[8]	YU Wenting, XUE Bin, ZHU Zhenshu, SHEN Ziqin, QIN Meng, WANG Wei, CAO Yi. Strong and Injectable Hydrogels Based on Multivalent Metal Ion-Peptide Cross-linking[J]. 高等学校化学研究, 2020, 36(5): 962-969.
[9]	ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei. Chemical Synthesis of Proteins Containing 300 Amino Acids[J]. 高等学校化学研究, 2020, 36(5): 733-747.
[10]	WANG Ruijue, SHENG Kai, HOU Yingqin, SUN Jialing, LU Hua. Tailoring Cationic Helical Polypeptides for Efficient Cytosolic Protein Delivery[J]. 高等学校化学研究, 2020, 36(1): 134-138.
[11]	CAI Qing, QIAO Chunyan, NING Jun, DING Xinxin, WANG Haoyang, ZHOU Yanmin. A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability[J]. 高等学校化学研究, 2019, 35(5): 908-915.
[12]	XU Jia, YU Xiaoxuan, ZHANG Yue, LIU Naizhang, GUAN Shuwen, WANG Liping. Extending Lifespan of Alzheimer's Mode Nematode CL4176 Using a Novel Bifunctional Peptide with Inhibition of β-Amyloid Aggregation and Anti-oxidation[J]. 高等学校化学研究, 2019, 35(2): 245-250.
[13]	GUAN Renquan, ZHAI Hongju, SUN Dewu, ZHANG Junkai, WANG Yan, LI Jiaxin. Effects of Ag Doping Content and Dispersion on the Photocatalytic and Antibacterial Properties in ZnO Nanoparticles[J]. 高等学校化学研究, 2019, 35(2): 271-276.
[14]	LIU Guifeng, CHEN Hongda, YU Shaonan, LI Xiaodong, WANG Zhenxin. CXCR4 Peptide Conjugated Au-Fe₂O₃Nanoparticles for Tumor-targeting Magnetic Resonance Imaging[J]. 高等学校化学研究, 2018, 34(4): 584-589.
[15]	XU Boxuan, DING Xiudong, WU Yachuang, CUI Lei, QIAN Ping, WANG Di, ZHAO Yanfang. Synthesis and Antibacterial Activity of Oxazolidinone Derivatives Containing Nitro Heteroaromatic Moiety[J]. 高等学校化学研究, 2018, 34(1): 51-56.

Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价