高等学校化学研究 ›› 1998, Vol. 14 ›› Issue (2): 153-159.
Electrochemical Probe of Hydroxylation of 4-Nitrophenol by Cytochrome C with Hydrogen Peroxide
ZHU Yi-min, WANG Jin-cheng, LI Jing-hong, DONG Shao-jun
- Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022
Electrochemical Probe of Hydroxylation of 4-Nitrophenol by Cytochrome C with Hydrogen Peroxide
ZHU Yi-min, WANG Jin-cheng, LI Jing-hong, DONG Shao-jun
- Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022
摘要： The reaction of hydrogen peroxide with cytochrome c makes them coupled to lead to the hydroxylation of 4-nitrophenol. In situ electrochemical probe was used to detect the hydroxylation of 4-nitrophenol, which can avoid the tedious extraction procedure, the loss of the active species and the interference of some colored substances in the detection of 4-nitrocatechol by spectroscopic method. The hydroxyl radical scavengers mannitol and sodium benzoate did not eliminate hydroxylation, but the inhibitory effect of uric acid on the hydroxylation lead to the formation of the ferryl species of the protein during the reaction. These studies suggest that the electrochemical probe might efficiently detect the trace 4-nitrocatechol from the onset of the hydroxylation reaction and thus provides a more sensitive tool.