高等学校化学研究 ›› 1998, Vol. 14 ›› Issue (2): 160-166.
FAB MS of Permethylated L-β-Aspartamido-Carbohydrates
WANG Tsong-hui1, CHEN T. F.2, Barofsky D. F.2
- 1. Department of Instrumental Analysis, Institute of Forensic Science, Beijing 100038;
2. Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331, USA
FAB MS of Permethylated L-β-Aspartamido-Carbohydrates
WANG Tsong-hui1, CHEN T. F.2, Barofsky D. F.2
- 1. Department of Instrumental Analysis, Institute of Forensic Science, Beijing 100038;
2. Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331, USA
摘要： Native glycopeptides are suitable to FAB mass spectrometric analysis due to their high polarity, low volatility and large molecular size. The deficiency in the FAB spectra of glycopeptides is the limited number of peaks and the low abundances of fragments. In order to obtain the information of carbohydrate sequence the permethylation of carbohydrate, a well known method, is recommended here. The L-β-aspartamido-carbohydrate from chicken egg albumin was chosen as the model compound of glycopeptides. Our experiments showed obvious carbohydrate-sequence in the spectra of permethylated aspartamido carbohydrates. The key conditions to perform the method are the methylation reagent and purification process for the permethylated derivatives.