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高等学校化学研究 ›› 1993, Vol. 9 ›› Issue (4): 288-295.

• Articles • 上一篇    下一篇

Sequence Analysis of Polypeptide by Mass Spectrometry

YANG Hou-jun, LI Xiao-ling, HU Xiao-yu, CHEN Yao-zu   

  1. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000
  • 收稿日期:1992-08-08 出版日期:1993-12-24 发布日期:2011-08-17
  • 通讯作者: YANG Hou-jun

Sequence Analysis of Polypeptide by Mass Spectrometry

YANG Hou-jun, LI Xiao-ling, HU Xiao-yu, CHEN Yao-zu   

  1. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000
  • Received:1992-08-08 Online:1993-12-24 Published:2011-08-17

摘要: The method described in this work provides a sensitive and fast technique for investigating the primary structure of peptides with molecular weight up to 3340 amu. Usually, the metastable ion kinetic energy spectra (MIKES) and collisional activated decomposition (CAD) spectra provide complementary information for the FAB mass spectra, the MIKES and CAD spectra generally contain high-mass sequence ions.

关键词: Peptide, Sequence analysis, Mass spectrometry.

Abstract: The method described in this work provides a sensitive and fast technique for investigating the primary structure of peptides with molecular weight up to 3340 amu. Usually, the metastable ion kinetic energy spectra (MIKES) and collisional activated decomposition (CAD) spectra provide complementary information for the FAB mass spectra, the MIKES and CAD spectra generally contain high-mass sequence ions.

Key words: Peptide, Sequence analysis, Mass spectrometry.