Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

doi:10.1007/s40242-024-4004-x

高等学校化学研究 ›› 2024, Vol. 40 ›› Issue (2): 237-254.doi: 10.1007/s40242-024-4004-x

Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

ZHANG Yi¹, ZHOU Bokai², LI Qiuning¹, JIN Mingshi², BAI Yu¹

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2. Department of Chemistry, Yanbian University, Yanji 133002, P. R. China

收稿日期:2024-01-07 修回日期:2024-03-07 出版日期:2024-04-01 发布日期:2024-03-27
通讯作者: BAI Yu yu.bai@pku.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos. 22074003, 22125401 and 22104127) and the National Key R&D Program of China (No. 2022YFC3400700).

Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

ZHANG Yi¹, ZHOU Bokai², LI Qiuning¹, JIN Mingshi², BAI Yu¹

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China;
2. Department of Chemistry, Yanbian University, Yanji 133002, P. R. China

Received:2024-01-07 Revised:2024-03-07 Online:2024-04-01 Published:2024-03-27
Contact: BAI Yu yu.bai@pku.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 22074003, 22125401 and 22104127) and the National Key R&D Program of China (No. 2022YFC3400700).

摘要/Abstract

摘要： Exosomes, extracellular vesicles (EVs) that play crucial roles in biological processes, have emerged as attractive targets for noninvasive disease diagnosis and monitoring. Mass spectrometry (MS) offers high sensitivity, high throughput, and excellent qualification and quantification capacity, which is the ideal approach for exosome analysis. Nanomaterials’ unique physiochemical properties, controllable morphology, and large surface area make them promising in biological sample pretreatment and detection. They contributed diverse functions to the exosome MS analysis, encompassing substrates for exosome isolation and cargos enrichment, matrices for laser desorption/ionization (LDI), read-out signals and supporters for signal amplification strategies, etc. In this review, recent progress in the applications of nanomaterial in the exosome MS analysis was summarized and a comprehensive discussion on the challenges and perspectives was proposed for the development of an advancing analysis approach of exosomes for the accurate diagnosis and monitoring of diseases.

关键词: Exosome, Nanomaterial, Enrichment, Matrix, Mass spectrometry

Abstract: Exosomes, extracellular vesicles (EVs) that play crucial roles in biological processes, have emerged as attractive targets for noninvasive disease diagnosis and monitoring. Mass spectrometry (MS) offers high sensitivity, high throughput, and excellent qualification and quantification capacity, which is the ideal approach for exosome analysis. Nanomaterials’ unique physiochemical properties, controllable morphology, and large surface area make them promising in biological sample pretreatment and detection. They contributed diverse functions to the exosome MS analysis, encompassing substrates for exosome isolation and cargos enrichment, matrices for laser desorption/ionization (LDI), read-out signals and supporters for signal amplification strategies, etc. In this review, recent progress in the applications of nanomaterial in the exosome MS analysis was summarized and a comprehensive discussion on the challenges and perspectives was proposed for the development of an advancing analysis approach of exosomes for the accurate diagnosis and monitoring of diseases.

Key words: Exosome, Nanomaterial, Enrichment, Matrix, Mass spectrometry

ZHANG Yi, ZHOU Bokai, LI Qiuning, JIN Mingshi, BAI Yu. Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry[J]. 高等学校化学研究, 2024, 40(2): 237-254.

ZHANG Yi, ZHOU Bokai, LI Qiuning, JIN Mingshi, BAI Yu. Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry[J]. Chemical Research in Chinese Universities, 2024, 40(2): 237-254.

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Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

Nanomaterial Assisted Exosome Analysis Using Mass Spectrometry

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