Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

doi:10.1007/s40242-022-2021-1

高等学校化学研究 ›› 2022, Vol. 38 ›› Issue (6): 1446-1452.doi: 10.1007/s40242-022-2021-1

Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

LIU Junxue, WANG Yufei, MA Wenyan, ZONG Siyu, LI Jiyang

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China

收稿日期:2022-01-19 出版日期:2022-12-01 发布日期:2022-12-06
通讯作者: LI Jiyang E-mail:lijiyang@jlu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos.21671075, 21621001) and the ″111″ Project of China(No.B17020).

Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

LIU Junxue, WANG Yufei, MA Wenyan, ZONG Siyu, LI Jiyang

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2022-01-19 Online:2022-12-01 Published:2022-12-06
Contact: LI Jiyang E-mail:lijiyang@jlu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos.21671075, 21621001) and the ″111″ Project of China(No.B17020).

摘要/Abstract

摘要： Biomass-carbon dots(named as B-CDs) have been successfully prepared via facile one-step hydrothermal reflux treatment at a low temperature from passion fruit peel. The as-prepared B-CDs had a uniform sphere morphology and size of 1-3 nm, which possessed rich nitrogen/oxygen-containing functional groups. B-CDs could imitate peroxidase and accelerate the reaction process of H₂O₂ oxidizing the substrate 3,3',5,5'-tetramethylbenzidine(TMB), which caused an obvious color change in the solution due to the oxidization of achromic TMB into blue oxidation product(ox-TMB) with an absorption peak at 652 nm. Furthermore, the product could be further reduced to native colorless TMB by the reducing agent glutathione or L-Cysteine. Thus, depending on the peroxidase-like properties of B-CDs, we developed a colorimetric approach to detecting both glutathione and L-Cysteine, which showed superior selective and sensitive detection towards glutathione and L-Cysteine in a linear range of 0-20 µmol/L. The limits of detection were 0.62 and 0.58 µmol/L, respectively.

关键词: Carbon dot, Biomass, Colorimetry, Glutathione, Cysteine

Abstract: Biomass-carbon dots(named as B-CDs) have been successfully prepared via facile one-step hydrothermal reflux treatment at a low temperature from passion fruit peel. The as-prepared B-CDs had a uniform sphere morphology and size of 1-3 nm, which possessed rich nitrogen/oxygen-containing functional groups. B-CDs could imitate peroxidase and accelerate the reaction process of H₂O₂ oxidizing the substrate 3,3',5,5'-tetramethylbenzidine(TMB), which caused an obvious color change in the solution due to the oxidization of achromic TMB into blue oxidation product(ox-TMB) with an absorption peak at 652 nm. Furthermore, the product could be further reduced to native colorless TMB by the reducing agent glutathione or L-Cysteine. Thus, depending on the peroxidase-like properties of B-CDs, we developed a colorimetric approach to detecting both glutathione and L-Cysteine, which showed superior selective and sensitive detection towards glutathione and L-Cysteine in a linear range of 0-20 µmol/L. The limits of detection were 0.62 and 0.58 µmol/L, respectively.

Key words: Carbon dot, Biomass, Colorimetry, Glutathione, Cysteine

LIU Junxue, WANG Yufei, MA Wenyan, ZONG Siyu, LI Jiyang. Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine[J]. 高等学校化学研究, 2022, 38(6): 1446-1452.

LIU Junxue, WANG Yufei, MA Wenyan, ZONG Siyu, LI Jiyang. Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine[J]. Chemical Research in Chinese Universities, 2022, 38(6): 1446-1452.

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Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

Biomass-based Carbon Dots as Peroxidase Mimics for Colorimetric Detection of Glutathione and L-Cysteine

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