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高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (5): 981-982.doi: 10.1007/s40242-020-0251-7

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Encapsulation and Release of Circulating Tumor Cells Using 3D DNA Hydrogels

GUO Zijian   

  1. State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China
  • 收稿日期:2020-08-04 修回日期:2020-08-07 出版日期:2020-10-01 发布日期:2020-10-01
  • 通讯作者: GUO Zijian E-mail:zguo@nju.edu.cn

Encapsulation and Release of Circulating Tumor Cells Using 3D DNA Hydrogels

GUO Zijian   

  1. State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P. R. China
  • Received:2020-08-04 Revised:2020-08-07 Online:2020-10-01 Published:2020-10-01
  • Contact: GUO Zijian E-mail:zguo@nju.edu.cn

摘要： To minimize the concomitant cell damage in the capture process of circulating tumor cells(CTCs) in conventional methods, Zuo et al. proposed to use aptamer-trigger-clamped hybridization chain reaction method to encapsulate CTCs by in-situ forming 3D DNA hydrogel. The 3D porous DNA hydrogel on cell membrane minimizes cell damage, and the CTCs can be readily released by the ATP triggered dis-assembly of DNA hydrogel for subsequent culture and live cell analysis. This work has been published online in the Nature Protocols in June 22, 2020.

Abstract: To minimize the concomitant cell damage in the capture process of circulating tumor cells(CTCs) in conventional methods, Zuo et al. proposed to use aptamer-trigger-clamped hybridization chain reaction method to encapsulate CTCs by in-situ forming 3D DNA hydrogel. The 3D porous DNA hydrogel on cell membrane minimizes cell damage, and the CTCs can be readily released by the ATP triggered dis-assembly of DNA hydrogel for subsequent culture and live cell analysis. This work has been published online in the Nature Protocols in June 22, 2020.