Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity

doi:10.1007/s40242-019-8406-0

高等学校化学研究 ›› 2019, Vol. 35 ›› Issue (4): 708-713.doi: 10.1007/s40242-019-8406-0

Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity

HUA Jinghan, ZHANG Hongbin, WU Hao, WANG Jiadong, HU Xueqin, YANG Jingwen

Department of Pharmaceutical Science and Engineering, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P. R. China

收稿日期:2018-12-24 修回日期:2019-05-06 出版日期:2019-08-01 发布日期:2019-08-01
通讯作者: YANG Jingwen E-mail:jwyang@hfut.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.81573399) and the Fundamental Research Funds for the Central Universities, China.

Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity

HUA Jinghan, ZHANG Hongbin, WU Hao, WANG Jiadong, HU Xueqin, YANG Jingwen

Department of Pharmaceutical Science and Engineering, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P. R. China

Received:2018-12-24 Revised:2019-05-06 Online:2019-08-01 Published:2019-08-01
Contact: YANG Jingwen E-mail:jwyang@hfut.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.81573399) and the Fundamental Research Funds for the Central Universities, China.

摘要/Abstract

摘要： Cyclodextrins(CDs) are widely used in food, pharmaceuticals, drug delivery, and chemical industries and in agriculture and environmental engineering. To improve the yield and selectivity of CDs, this work presented a facile, scalable and efficient enzymatic synthesis of β-CD from starch using β-cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from Bacillus cereus. First, we found that the pretreatment of starch dramatically influenced CDs yield that was related to the structure and molecular weight of the substrate starch. Second, alcohol solvents influenced the yield and product selectivity of CDs; tertiary alcohols enhanced CDs yield(from 54.95% to 68.21%) and secondary alcohols increased the product selectivity(β-CD/γ-CD changed from 6.25 to 8.05). Fluorescence quenching analysis showed that the binding constants and entropy of the solvents influenced the yield and product selectivity, respectively. In conclusion, the results demonstrate that this study provides a promising method for the industrial production of β-CD.

关键词: β-Cyclodextrin glycosyltransferase, Alcohol solvent, Bacillus cereus, Cyclodextrin

Abstract: Cyclodextrins(CDs) are widely used in food, pharmaceuticals, drug delivery, and chemical industries and in agriculture and environmental engineering. To improve the yield and selectivity of CDs, this work presented a facile, scalable and efficient enzymatic synthesis of β-CD from starch using β-cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from Bacillus cereus. First, we found that the pretreatment of starch dramatically influenced CDs yield that was related to the structure and molecular weight of the substrate starch. Second, alcohol solvents influenced the yield and product selectivity of CDs; tertiary alcohols enhanced CDs yield(from 54.95% to 68.21%) and secondary alcohols increased the product selectivity(β-CD/γ-CD changed from 6.25 to 8.05). Fluorescence quenching analysis showed that the binding constants and entropy of the solvents influenced the yield and product selectivity, respectively. In conclusion, the results demonstrate that this study provides a promising method for the industrial production of β-CD.

Key words: β-Cyclodextrin glycosyltransferase, Alcohol solvent, Bacillus cereus, Cyclodextrin

HUA Jinghan, ZHANG Hongbin, WU Hao, WANG Jiadong, HU Xueqin, YANG Jingwen. Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity[J]. 高等学校化学研究, 2019, 35(4): 708-713.

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Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity

Discovery and Characterization of a Novel Method for Effective Improvement of Cyclodextrin Yield and Product Specificity

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