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高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 157-163.doi: 10.1007/s40242-019-0030-5

• Reviews • 上一篇    下一篇

CRISPR-Cas System for RNA Detection and Imaging

CHEN Siyu, WANG Rujia, LEI Chunyang, NIE Zhou   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, P. R. China
  • 收稿日期:2019-10-29 修回日期:2019-11-25 出版日期:2020-04-01 发布日期:2020-03-18
  • 通讯作者: NIE Zhou E-mail:niezhou.hnu@gmail.com,niezhou@hnu.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China(Nos.21725503, 21575038) and the Young Top-notch Talent for Ten Thousand Talent Program of China.

CRISPR-Cas System for RNA Detection and Imaging

CHEN Siyu, WANG Rujia, LEI Chunyang, NIE Zhou   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, P. R. China
  • Received:2019-10-29 Revised:2019-11-25 Online:2020-04-01 Published:2020-03-18
  • Contact: NIE Zhou E-mail:niezhou.hnu@gmail.com,niezhou@hnu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos.21725503, 21575038) and the Young Top-notch Talent for Ten Thousand Talent Program of China.

摘要: The system of clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated endonucleases(Cas) have been widely used in gene editing, disease treatment, molecular diagnosis and chromosome imaging. On account of the programmable target recognition of CRISPR-Cas system and the specific targeting function toward RNA of type VI class II Cas proteins, CRISPR-Cas system has been deployed as RNA recognition and detection tools, exhibiting promising application potentials in the field of RNA detection and imaging. In this review, we summarize the latest research progresses as well as development prospects of CRISPR-Cas system in RNA diagnosis and live cell RNA imaging.

关键词: Clustered regularly interspaced short palindromic repeats-associated endonucleases (CRISPR-Cas), RNA detection, RNA imaging, Collateral cleavage

Abstract: The system of clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated endonucleases(Cas) have been widely used in gene editing, disease treatment, molecular diagnosis and chromosome imaging. On account of the programmable target recognition of CRISPR-Cas system and the specific targeting function toward RNA of type VI class II Cas proteins, CRISPR-Cas system has been deployed as RNA recognition and detection tools, exhibiting promising application potentials in the field of RNA detection and imaging. In this review, we summarize the latest research progresses as well as development prospects of CRISPR-Cas system in RNA diagnosis and live cell RNA imaging.

Key words: Clustered regularly interspaced short palindromic repeats-associated endonucleases (CRISPR-Cas), RNA detection, RNA imaging, Collateral cleavage