A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

doi:10.1007/s40242-015-5135-x

高等学校化学研究 ›› 2015, Vol. 31 ›› Issue (5): 781-786.doi: 10.1007/s40242-015-5135-x

A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

ZHAO Liqiang, LIU Jinyuan, SONG Dongmin, WANG Xiangxiang, TAI Feifei, XU Haijin, QIAO Mingqiang

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, P. R. China

收稿日期:2015-04-03 修回日期:2015-07-15 出版日期:2015-10-01 发布日期:2015-07-27
通讯作者: QIAO Mingqiang, E-mail: qiaomq@nankai.edu.cn E-mail:qiaomq@nankai.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.31170066) and the Tianjin Key Research Program of Application Foundation and Advanced Technology, China(No.12JCZDJC22600).

A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

ZHAO Liqiang, LIU Jinyuan, SONG Dongmin, WANG Xiangxiang, TAI Feifei, XU Haijin, QIAO Mingqiang

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, P. R. China

Received:2015-04-03 Revised:2015-07-15 Online:2015-10-01 Published:2015-07-27
Contact: QIAO Mingqiang, E-mail: qiaomq@nankai.edu.cn E-mail:qiaomq@nankai.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.31170066) and the Tianjin Key Research Program of Application Foundation and Advanced Technology, China(No.12JCZDJC22600).

摘要/Abstract

摘要：

Hydrophobins are a type of small amphipathic proteins with a unique self-assembly property, which can be used to modify material surfaces and adsorb enzymes, antibodies and even cells. In this study, a fusion protein consisting of hydrophobin HGFI and green fluorescent protein(GFP) was successfully obtained from Pichia patoris (P. pastoris). Water contact angle(WCA) measurement proves that the wettability of the surfaces of different materials was changed. We further demonstrated the self-assembly ability of HGFI-GFP, which can be used to disperse the multi-walled carbon nanotubes(MWCNTs). Finally, the adsorption of HGFI-GFP onto the surface of the tissue engineering material poly(ε-caprolactone)(PCL) was evaluated by detecting the fluorescence of the fusion protein itself. The resalt demonstrates that both the basic self-assembly activity of the HGFI domain and the functional activity of the GFP domain were still remained.

关键词: Self-assembly, Hydrophobin, Fusion protein, Dispersion, Modification

Abstract:

Key words: Self-assembly, Hydrophobin, Fusion protein, Dispersion, Modification

ZHAO Liqiang, LIU Jinyuan, SONG Dongmin, WANG Xiangxiang, TAI Feifei, XU Haijin, QIAO Mingqiang. A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI[J]. 高等学校化学研究, 2015, 31(5): 781-786.

ZHAO Liqiang, LIU Jinyuan, SONG Dongmin, WANG Xiangxiang, TAI Feifei, XU Haijin, QIAO Mingqiang. A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI[J]. Chemical Research in Chinese Universities, 2015, 31(5): 781-786.

参考文献

[1] Wessels J. G., de Vries O. M., Asgeirsdottir S. A., Springer J., Journal of General Microbiology, 1991, 137, 2439
[2] Wessels J. G., Advances in Microbial Physiology, 1997, 38, 43
[3] Bayry J., Aimanianda V., Guijarro J. I., Sunde M., Latge J. P., PLoS Pathogens, 2012, 8
[4] Kershaw M. J., Thornton C. R., Wakley G. E., Talbot N. J., Molecular Microbiology, 2005, 56, 117
[5] Linder M. B., Current Opinion in Colloid & Interface Science, 2009, 14, 356
[6] Yu L., Zhang B., Szilvay G. R., Sun R., Janis J., Wang Z., Feng S., Xu H., Linder M. B., Qiao M., Microbiology-Sgm, 2008, 154, 1677
[7] Misra R., Li J., Cannon G. C., Morgan S. E., Biomacromolecules, 2006, 7, 1463
[8] De Stefano L., Rea I., Armenante A., Giardina P., Giocondo M., Rendina I., Langmuir, 2007, 23, 7921
[9] Zhao Z. X., Wang H. C., Qin X., Wang X. S., Qiao M. Q., Anzai J. I., Chen Q., Colloids and Surfaces B-Biointerfaces, 2009, 71, 104
[10] Corvis Y., Walcarius A., Rink R., Mrabet N. T., Rogalska E. Analytical Chemistry, 2005, 77, 1626
[11] Wang Z. F., Huang Y. J., Niu B. L., Li S., Wang D. D., Xu H. J., Qiao M. Q., Chem. J. Chinese Universities, 2010, 31(10), 2053
[12] Hou S., Yang K., Qin M., Feng X. Z., Guan L., Yang Y., Wang C., Biosensors and Bioelectronics, 2008, 24, 912
[13] Niu B., Huang Y., Zhang S., Wang D., Xu H., Kong D., Qiao M., Protein Expression Purification, 2012, 83, 92
[14] Huang Y., Zhang S., Niu B., Wang D., Wang Z., Feng S., Xu H., Kong D., Qiao M., Colloids and Surfaces B-Biointerfaces, 2013, 101, 364
[15] Boeuf S., Throm T., Gutt B., Strunk T., Hoffmann M., Seebach E., Muehlberg L., Brocher J., Gotterbarm T., Wenzel W., Fischer R., Richter W., Acta Biomaterialia, 2012, 8, 1042
[16] Schagger H., Nature Protocols, 2006, 1, 17
[17] Swayne T. C., Boldogh I. R., Pon L. A., Cytoskeleton Methods and Protocols, 2009, 586, 179
[18] Wang Z., Feng S., Huang Y., Li S., Xu H., Zhang X., Bai Y., Qiao M., Protein Expression and Purification, 2010, 72, 19
[19] Wang Z., Wang Y., Huang Y., Li S., Feng S., Xu H., Qiao M., Carbon, 2010, 48, 2896
[20] Vizcaino-caston I., Wyre C., Overton T. W., Biotechnology Letters, 2012, 34, 178
[21] Wang H., Nakata E., Hamachi I., Chembiochem., 2009, 10, 2565
[22] Pilbrough W., Munro T. P., Gray P., PLoS One, 2009, 4, e8432
[23] Jones J. J., Bridges A. M., Fosberry A. P., Gardner S., Lowes R. R., Newby R. R., James P. J., Hall R. M., Jenkins O., Journal of Biotechnology, 2004, 109, 204
[24] Zykwinska A., Guillemette T., Bouchara J. P., Cuenot S., Biochimica et Biophysica Acta(BBA)——Proteins and Proteomics, 2014, 1844, 1233
[25] Zangi R., de Vocht M. L., Robillard G. T., Mark A., Biophysical Journal, 2002, 83, 113
[26] Wang X., Graveland-Bikker J. F., DeKruif C. G., Robillard G. T., Protein Science, 2004, 13, 815
[27] Pedersen M. H., Borodina I., Moresco J. L., Svendsen W. E., Frisvad J. C., Sondergaard I., Applied Microbiology and Biotechnology, 2011, 90, 1926
[28] Askolin S., Linder M., Scholtmeijer K., Tenkanen M., Penttila M., de Vocht M. L., Wosten H. A. B., Biomacromolecules, 2006, 7, 1295
[29] Panjideh H., Coelho V., Dernedde J., Fuchs H., Keilholz U., Thiel E., Deckert P. M., Bioprocess Biosystems Engineering, 2008, 31, 563
[30] Qin M., Wang L. K., Feng X. Z., Yang Y. L., Wang R., Wang C., Yu L., Shao B., Qiao M. Q., Langmuir, 2007, 23, 4469
[31] Ding G. B., Liu H. Y., Wang Y., Lu Y. Y., Wu Y., Guo Y., Xu L., Chem. Res. Chinese Universities, 2013, 29(1), 105
[32] Winkler M., Raupp Y. S., Kohl L. A., Wagner H. E., Meier M. A. R., Macromolecules, 2014, 47, 2846
[33] Wang X., Wang H., Huang Y., Zhao Z., Qin X., Wang Y., Miao Z., Chen Q., Qiao M., Biosensors & Bioelectronics, 2010, 26, 1108
[34] Palomo J. M., Penas M. M., Fernandez-Lorente G., Mateo C., Pisabarro A. G., Fernandez-Lafuente R., Ramirez L., Guisan J. M., Biomacromolecules, 2003, 4, 206
[35] Chen Z., Zhang J., Guo Y., Zhang H., Cao J., Xu Q., Wang S., Wang B., Liu Z., Chem. Res. Chinese Universities, 2014, 30(4), 696
[36] Dai H., Xiao D. L., He H., Yuan D. H., Zhang C., Microchimica Acta, 2014, 182, 895

A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

A Visualized Fusion Protein Based on Self-assembly Hydrophobin HGFI

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