Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights

高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (6): 1072-1077.

Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights

LU Gui-qian^1,2, ZUO Hua-jiang^1,2, DONG Wei-min¹, WU Ding-cai¹ and FU Ruo-wen^1,2*

1. Key Laboratory for Polymeric Composite and Functional Materials, Ministry of Education,?
2. Design and Synthesis of New Polymer Materials and Application Laboratory, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China

收稿日期:2011-01-13 修回日期:2011-03-23 出版日期:2011-11-25 发布日期:2011-11-07
通讯作者: FU Ruo-wen E-mail:cesfrw@mail.sysu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.50673101) and the Project of Department of Education of Guangdong Province, China(No. cgzhzd0901).

Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights

LU Gui-qian^1,2, ZUO Hua-jiang^1,2, DONG Wei-min¹, WU Ding-cai¹ and FU Ruo-wen^1,2*

1. Key Laboratory for Polymeric Composite and Functional Materials, Ministry of Education,?
2. Design and Synthesis of New Polymer Materials and Application Laboratory, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China

Received:2011-01-13 Revised:2011-03-23 Online:2011-11-25 Published:2011-11-07
Contact: FU Ruo-wen E-mail:cesfrw@mail.sysu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.50673101) and the Project of Department of Education of Guangdong Province, China(No. cgzhzd0901).

摘要/Abstract

摘要： Antibacterial polymers of dimethylaminoethyl methacrylate benzyl ammonium chloride(DMAEMA-BC) with various molecular weights(Mws) were prepared under controlling radical polymerization conditions. The Mws of these polymers were determined by means of static multiangle laser light scattering and viscosity method. A Mark-Houwink equation was established to be [η]=0.154M0.764 for the Mw evaluation of poly(DMAEMA-BC)s. The effects of the Mws of these poly(DMAEMA-BC)s on their antibacterial activities against E. coli and S. aureus were investigated by various methods including viable cell counting, electrical conductivity titration, intracellular constituent leakage tests and electron microscopy. Our results indicate that the antibacterial efficiency of DMAEMA-BC was significantly enhanced after the monomers were polymerized into a polymer and increased obviously with the Mws，as a result of the increase of charge density. Further investigation of the molecular basis underlying the anti-bacterial role of these polymers revealed that poly(DMAEMA-BC) promoted the release of potassium ion from the membrane of bacterial cells and the release increased significantly with the Mws of the polymers used.

关键词: Polymeric quaternary ammonium salt, Antibacterial activity, Molecular weight

Abstract: Antibacterial polymers of dimethylaminoethyl methacrylate benzyl ammonium chloride(DMAEMA-BC) with various molecular weights(Mws) were prepared under controlling radical polymerization conditions. The Mws of these polymers were determined by means of static multiangle laser light scattering and viscosity method. A Mark-Houwink equation was established to be [η]=0.154M0.764 for the Mw evaluation of poly(DMAEMA-BC)s. The effects of the Mws of these poly(DMAEMA-BC)s on their antibacterial activities against E. coli and S. aureus were investigated by various methods including viable cell counting, electrical conductivity titration, intracellular constituent leakage tests and electron microscopy. Our results indicate that the antibacterial efficiency of DMAEMA-BC was significantly enhanced after the monomers were polymerized into a polymer and increased obviously with the Mws，as a result of the increase of charge density. Further investigation of the molecular basis underlying the anti-bacterial role of these polymers revealed that poly(DMAEMA-BC) promoted the release of potassium ion from the membrane of bacterial cells and the release increased significantly with the Mws of the polymers used.

Key words: Polymeric quaternary ammonium salt, Antibacterial activity, Molecular weight

LU Gui-qian, ZUO Hua-jiang, DONG Wei-min, WU Ding-cai and FU Ruo-wen*. Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights[J]. 高等学校化学研究, 2011, 27(6): 1072-1077.

LU Gui-qian, ZUO Hua-jiang, DONG Wei-min, WU Ding-cai and FU Ruo-wen*. Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights[J]. Chemical Research in Chinese Universities, 2011, 27(6): 1072-1077.

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Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights

Synthesis and Antibacterial Action of Poly(DMAEMA-BC) with Various Molecular Weights

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