Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (4): 604-607.

Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library

REN Hui¹, XIONG Xin-hui², JIANG Tao³, ZHANG Yang-de^1*, WEI Zhong-hang³, SONG Xiang-wei², GUAN Shu-wen², WANG Yan³ and WANG Li-ping^2,3*

1. Center of Hepato-cholangio-intestinal Surgery of Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, P. R. China;
2. College of Life Science, Jilin University, Changchun 130012, P. R. China;
3. Department of General Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China

收稿日期:2010-03-15 修回日期:2010-04-13 出版日期:2010-07-25 发布日期:2010-10-01
通讯作者: WANG Li-ping, E-mail: wanglp@jlu.edu.cn; ZHANG Yang-de, E-mail: zyd@2118.cn

Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library

REN Hui¹, XIONG Xin-hui², JIANG Tao³, ZHANG Yang-de^1*, WEI Zhong-hang³, SONG Xiang-wei², GUAN Shu-wen², WANG Yan³ and WANG Li-ping^2,3*

1. Center of Hepato-cholangio-intestinal Surgery of Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, P. R. China;
2. College of Life Science, Jilin University, Changchun 130012, P. R. China;
3. Department of General Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China

Received:2010-03-15 Revised:2010-04-13 Online:2010-07-25 Published:2010-10-01
Contact: WANG Li-ping, E-mail: wanglp@jlu.edu.cn; ZHANG Yang-de, E-mail: zyd@2118.cn

摘要/Abstract

摘要： In order to provide the structure information for designing new exendin-4 analogues, a phage display peptide library was screened by targeting the N-terminal extracellular domain of GLP-1R(nGLP-1R). After four rounds of selection, nine sequences were obtained, four of them have higher affinity for nGLP-1R than the others. We chose two of them named X and Y peptides. Islet β-cell proliferation assay suggested that X and Y peptides didn’t have any activity to increase islet β-cell proliferation. In other words, X and Y peptides were not agonists to GLP-1R. However, the conservative motifs of X and Y peptides provided us useful information to design new exendin-4 analogues.

关键词: GLP-1 receptor, Phage display peptide library, Exendin-4

Abstract: In order to provide the structure information for designing new exendin-4 analogues, a phage display peptide library was screened by targeting the N-terminal extracellular domain of GLP-1R(nGLP-1R). After four rounds of selection, nine sequences were obtained, four of them have higher affinity for nGLP-1R than the others. We chose two of them named X and Y peptides. Islet β-cell proliferation assay suggested that X and Y peptides didn’t have any activity to increase islet β-cell proliferation. In other words, X and Y peptides were not agonists to GLP-1R. However, the conservative motifs of X and Y peptides provided us useful information to design new exendin-4 analogues.

Key words: GLP-1 receptor, Phage display peptide library, Exendin-4

REN Hui, XIONG Xin-hui, JIANG Tao, ZHANG Yang-de*, WEI Zhong-hang, .... Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library[J]. 高等学校化学研究, 2010, 26(4): 604-607.

REN Hui, XIONG Xin-hui, JIANG Tao, ZHANG Yang-de*, WEI Zhong-hang, .... Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library[J]. Chemical Research in Chinese Universities, 2010, 26(4): 604-607.

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Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library

Screening and Identification of a Targeting Peptide to nGLP-1R from Phage Display Peptide Library

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