Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (4): 596-603.

Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

LIU Tao^1,2, JIN Ying-hua², ZHANG Yu¹, CHANG Ming¹, WANG Dan-ping¹ and HU Lin-sen^1*

1. Department of Neurology, First Hospital,
2. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, P. R. China

收稿日期:2009-09-02 修回日期:2010-02-02 出版日期:2010-07-25 发布日期:2010-10-01
通讯作者: HU Lin-sen, E-mail: hulinsen@hotmail.com

Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

LIU Tao^1,2, JIN Ying-hua², ZHANG Yu¹, CHANG Ming¹, WANG Dan-ping¹ and HU Lin-sen^1*

1. Department of Neurology, First Hospital,
2. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, P. R. China

Received:2009-09-02 Revised:2010-02-02 Online:2010-07-25 Published:2010-10-01
Contact: HU Lin-sen, E-mail: hulinsen@hotmail.com

摘要/Abstract

摘要： Parkinson’s disease(PD) is a common neurodegenerative disorder whose primary pathology features are the degeneration of dopaminergic neurons in the substantia nigra pars compacta(SNc) and the presence of eosinophilic inclusions called Lewy body in the cytoplasm of the remained neurons. Growing evidence suggests that dysfunction of the ubiquitin-proteasome system(UPS) is involved in the etiopathogenesis of PD. In order to investigate the pathogenetic mechanism of ubiquitin-proteasome dysfunction in PD, 2D-differential gel electrophoresis(2D-DIGE) and MALDI-TOF Pro MS were used to determine the proteins, which were differentially expressed, in PC12 cells that had undergone a synthetic proteasomal inhibitor PSI(10 μmol/L) treatment for 24 h. Forty-six protein spots were differentially expressed in response to PSI administration, of which 34 were increased and 12 decreased. Six of these were identified as molecular charperones: endoplasmin precursor(GRP94), heat shock protein 105(HSP105), HSC-70-psl, glucose ruglated protein 75(GRP75), glucose ruglated protein 58(GRP58) and heat shock 27000 protein 1(HSP27). The results suggest that the molecular chaperones play an important role in the PD model induced by proteasomal inhibitor.

关键词: Ubiquitin-proteasome system(UPS), Molecular chaperone, Parkinson’s disease(PD)

Abstract: Parkinson’s disease(PD) is a common neurodegenerative disorder whose primary pathology features are the degeneration of dopaminergic neurons in the substantia nigra pars compacta(SNc) and the presence of eosinophilic inclusions called Lewy body in the cytoplasm of the remained neurons. Growing evidence suggests that dysfunction of the ubiquitin-proteasome system(UPS) is involved in the etiopathogenesis of PD. In order to investigate the pathogenetic mechanism of ubiquitin-proteasome dysfunction in PD, 2D-differential gel electrophoresis(2D-DIGE) and MALDI-TOF Pro MS were used to determine the proteins, which were differentially expressed, in PC12 cells that had undergone a synthetic proteasomal inhibitor PSI(10 μmol/L) treatment for 24 h. Forty-six protein spots were differentially expressed in response to PSI administration, of which 34 were increased and 12 decreased. Six of these were identified as molecular charperones: endoplasmin precursor(GRP94), heat shock protein 105(HSP105), HSC-70-psl, glucose ruglated protein 75(GRP75), glucose ruglated protein 58(GRP58) and heat shock 27000 protein 1(HSP27). The results suggest that the molecular chaperones play an important role in the PD model induced by proteasomal inhibitor.

Key words: Ubiquitin-proteasome system(UPS), Molecular chaperone, Parkinson’s disease(PD)

LIU Tao, JIN Ying-hua, ZHANG Yu, CHANG Ming, WANG Dan-ping and HU Lin-sen*. Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI[J]. 高等学校化学研究, 2010, 26(4): 596-603.

LIU Tao, JIN Ying-hua, ZHANG Yu, CHANG Ming, WANG Dan-ping and HU Lin-sen*. Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI[J]. Chemical Research in Chinese Universities, 2010, 26(4): 596-603.

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Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

Differential Expression of Molecular Chaperones in PC12 Cells Treated with PSI

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