Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

doi:10.1007/s40242-021-1104-8

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 522-527.doi: 10.1007/s40242-021-1104-8

Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

FENG Zhendong^1,2, ZHANG Xiaopei^1,2, LIU Na^1,2, WANG Yue^1,2, ZHOU Ziyi^1,2, GLEBOV O. Oleg^1,3, WU Tong^1,2

1. Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, P. R. China;
2. Qingdao Medical College, Qingdao University, Qingdao 266071, P. R. China;
3. Department of Old Age Psychiatry, The Institute of Psychiatry, Psychology & Neuroscience, King's College London, De Crespigny Park, Denmark Hill, London SE5 8AF, UK

收稿日期:2021-03-14 修回日期:2021-04-28 出版日期:2021-06-01 发布日期:2021-05-31
通讯作者: WU Tong E-mail:twu@qdu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China(No.82001970) and the Startup Funds from Qingdao University, China (No.DC2000002357).

Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

FENG Zhendong^1,2, ZHANG Xiaopei^1,2, LIU Na^1,2, WANG Yue^1,2, ZHOU Ziyi^1,2, GLEBOV O. Oleg^1,3, WU Tong^1,2

1. Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, P. R. China;
2. Qingdao Medical College, Qingdao University, Qingdao 266071, P. R. China;
3. Department of Old Age Psychiatry, The Institute of Psychiatry, Psychology & Neuroscience, King's College London, De Crespigny Park, Denmark Hill, London SE5 8AF, UK

Received:2021-03-14 Revised:2021-04-28 Online:2021-06-01 Published:2021-05-31
Contact: WU Tong E-mail:twu@qdu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China(No.82001970) and the Startup Funds from Qingdao University, China (No.DC2000002357).

摘要/Abstract

摘要： We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites. In this case, nonwoven mats of uniaxially aligned poly(caprolactone)(PCL) nanofibers were firstly cut into several small pieces with fixed fiber lengths of 25, 50 and 100 μm, respectively, using a cryotome. A tissuelyser was employed to homogenize and disperse the short nanofibers to a homogeneous suspension. By tuning treatment duration from 100 s to 400 s, the temperature of the suspension was brought close to the melting point of PCL. As such, the short nanofibers were welded at their cross points while the fibers far away from the cross points remain the original structures. We showed that the viability of neuroblastoma SH-SY5Y cells and their neurite outgrowth and extension were enhanced with the use of such welded short nanofibers. Taken together, this study provides a simple way to generate injectable welded nanofibers, holding potential in affecting neurite outgrowth and extension for nerve repair, in particular, in the central nervous system.

关键词: Electrospinning, Nanofiber, Welding, Injectable, Neurite outgrowth

Abstract: We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites. In this case, nonwoven mats of uniaxially aligned poly(caprolactone)(PCL) nanofibers were firstly cut into several small pieces with fixed fiber lengths of 25, 50 and 100 μm, respectively, using a cryotome. A tissuelyser was employed to homogenize and disperse the short nanofibers to a homogeneous suspension. By tuning treatment duration from 100 s to 400 s, the temperature of the suspension was brought close to the melting point of PCL. As such, the short nanofibers were welded at their cross points while the fibers far away from the cross points remain the original structures. We showed that the viability of neuroblastoma SH-SY5Y cells and their neurite outgrowth and extension were enhanced with the use of such welded short nanofibers. Taken together, this study provides a simple way to generate injectable welded nanofibers, holding potential in affecting neurite outgrowth and extension for nerve repair, in particular, in the central nervous system.

Key words: Electrospinning, Nanofiber, Welding, Injectable, Neurite outgrowth

FENG Zhendong, ZHANG Xiaopei, LIU Na, WANG Yue, ZHOU Ziyi, GLEBOV O. Oleg, WU Tong. Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers[J]. 高等学校化学研究, 2021, 37(3): 522-527.

FENG Zhendong, ZHANG Xiaopei, LIU Na, WANG Yue, ZHOU Ziyi, GLEBOV O. Oleg, WU Tong. Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers[J]. Chemical Research in Chinese Universities, 2021, 37(3): 522-527.

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Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers

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