Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

doi:10.1007/s40242-021-1129-z

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 578-583.doi: 10.1007/s40242-021-1129-z

Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

CHEN Zhijun^1,2, HAO Ming¹, QIAN Xiaoming¹, CHEN Wenyang¹, ZENG Ming¹, HUANG Juan², LI Ruixin^3,4, FAN Jintu¹, LIU Yanbo²

1. School of Textile Science and Engineering, State Key Laboratory of Separation Membranes and Membrane Processes, Tiangong University, Tianjin 300387, P. R. China;
2. School of Textile Science and Engineering, Key Laboratory of Textile Fiber and Product, Ministry of Education, Wuhan Textile University, Wuhan 430200, P. R. China;
3. Central Laboratory, Tianjin Stomatological Hospital, Tianjin 300041, P. R. China;
4. Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, Tianjin 300041, P. R. China

收稿日期:2021-03-16 修回日期:2021-04-30 出版日期:2021-06-01 发布日期:2021-05-08
通讯作者: LIU Yanbo E-mail:yanboliu@gmail.com
基金资助:
This work was supported by the National Natural Science Foundation of China (No.51973168).

Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

CHEN Zhijun^1,2, HAO Ming¹, QIAN Xiaoming¹, CHEN Wenyang¹, ZENG Ming¹, HUANG Juan², LI Ruixin^3,4, FAN Jintu¹, LIU Yanbo²

1. School of Textile Science and Engineering, State Key Laboratory of Separation Membranes and Membrane Processes, Tiangong University, Tianjin 300387, P. R. China;
2. School of Textile Science and Engineering, Key Laboratory of Textile Fiber and Product, Ministry of Education, Wuhan Textile University, Wuhan 430200, P. R. China;
3. Central Laboratory, Tianjin Stomatological Hospital, Tianjin 300041, P. R. China;
4. Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, Tianjin 300041, P. R. China

Received:2021-03-16 Revised:2021-04-30 Online:2021-06-01 Published:2021-05-08
Contact: LIU Yanbo E-mail:yanboliu@gmail.com
Supported by:
This work was supported by the National Natural Science Foundation of China (No.51973168).

摘要/Abstract

摘要： In this study, orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolactone(PCL) scaffolds by the near-field direct-writing melt electrospinning(NFDWMES) technology. Based on the optimal process parameters, the PCL scaffolds with different thicknesses, gaps and structures were manufactured and the corresponding hydrophilicities were characterized. The PCL scaffolds were modified by chitosan (CS) and hyaluronic acid(HA) to improve biocompatibility and hydrophilicity. Both Fourier transform infrared spectroscopy(FTIR) analysis and antibacterial experimental results show that the chitosan and hyaluronic acid adhere to the surface of PCL scaffolds, sugges-ting that the modification plays a positive role in biocompatibility and antibacterial effect. The PCL scaffolds were then employed as a carrier to culture cells. The morphology and distribution of the cells observed by a fluorescence microscope demonstrate that the mo-dified PCL scaffolds have good biocompatibility, and the porous structure of the scaffolds is conducive to adhesion and deep growth of cells.

关键词: Melt electrospinning, Near-field direct-writing, Tissue engineering scaffold, Polycaprolactone, Modification

Abstract: In this study, orthogonal experiments were designed to explore the optimal process parameters for preparing polycaprolactone(PCL) scaffolds by the near-field direct-writing melt electrospinning(NFDWMES) technology. Based on the optimal process parameters, the PCL scaffolds with different thicknesses, gaps and structures were manufactured and the corresponding hydrophilicities were characterized. The PCL scaffolds were modified by chitosan (CS) and hyaluronic acid(HA) to improve biocompatibility and hydrophilicity. Both Fourier transform infrared spectroscopy(FTIR) analysis and antibacterial experimental results show that the chitosan and hyaluronic acid adhere to the surface of PCL scaffolds, sugges-ting that the modification plays a positive role in biocompatibility and antibacterial effect. The PCL scaffolds were then employed as a carrier to culture cells. The morphology and distribution of the cells observed by a fluorescence microscope demonstrate that the mo-dified PCL scaffolds have good biocompatibility, and the porous structure of the scaffolds is conducive to adhesion and deep growth of cells.

Key words: Melt electrospinning, Near-field direct-writing, Tissue engineering scaffold, Polycaprolactone, Modification

CHEN Zhijun, HAO Ming, QIAN Xiaoming, CHEN Wenyang, ZENG Ming, HUANG Juan, LI Ruixin, FAN Jintu, LIU Yanbo. Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning[J]. 高等学校化学研究, 2021, 37(3): 578-583.

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Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning

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