Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures

doi:10.1007/s40242-020-0110-6

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 301-306.doi: 10.1007/s40242-020-0110-6

Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures

LIU Shuo, YANG Mingjie, GUO Weiwei

College of Chemistry, Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, P. R. China

收稿日期:2019-12-25 修回日期:2020-03-02 出版日期:2020-04-01 发布日期:2020-03-02
通讯作者: GUO Weiwei E-mail:weiweiguo@nankai.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.21505078, 21874076), the Natural Science Foundation of Tianjin City, China(No.18JCZDJC37800), the Fundamental Research Funds for Central Universities(China) and the National Program for Support of Top-notch Young Professionals, China.

Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures

LIU Shuo, YANG Mingjie, GUO Weiwei

College of Chemistry, Research Centre for Analytical Sciences, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, P. R. China

Received:2019-12-25 Revised:2020-03-02 Online:2020-04-01 Published:2020-03-02
Contact: GUO Weiwei E-mail:weiweiguo@nankai.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21505078, 21874076), the Natural Science Foundation of Tianjin City, China(No.18JCZDJC37800), the Fundamental Research Funds for Central Universities(China) and the National Program for Support of Top-notch Young Professionals, China.

摘要/Abstract

摘要： Metal-organic frameworks(MOFs)-based nanozyme plays an important role in biosensing, therapy and catalysis. In this study, the effects of single-stranded DNA(ssDNA) with programmable sequences and its complementary DNA(T_DNA) on the intrinsic peroxidase-like activity of hemin loaded MOFs(UiO-66-NH₂), denoted as hemin@UiO-66-NH₂, were investigated. The hemin@UiO-66-NH₂ exhibited improved catalytic activity compared with free hemin. However, the catalytic activity is inhibited in the presence of ssDNA, as ssDNA can be adsorbed by MOFs and therefore protected the active sites from contact with substrates. Upon the addition of the T_DNA, double-stranded DNA(dsDNA) was formed and detached from the MOFs, resulting in the recovery of catalytic activity. Sequentially adding ssDNA or its complementary DNA strands can achieve the reversible regulation of the catalytic activity of MOFs nanozymes. Moreover, the DNA hybridization-based regulation was further applied to a cascaded catalytic system composed of the nanozyme, hemin@UiO-66-NH₂, and glucose oxidase. These nanozyme based programmable and reversibly regulated catalytic systems may have potential applications in future smart biosensing and catalysis systems.

关键词: Nanozyme, Catalytic metal-organic framework, Hemin, DNA hybridization, Dynamic modification

Abstract: Metal-organic frameworks(MOFs)-based nanozyme plays an important role in biosensing, therapy and catalysis. In this study, the effects of single-stranded DNA(ssDNA) with programmable sequences and its complementary DNA(T_DNA) on the intrinsic peroxidase-like activity of hemin loaded MOFs(UiO-66-NH₂), denoted as hemin@UiO-66-NH₂, were investigated. The hemin@UiO-66-NH₂ exhibited improved catalytic activity compared with free hemin. However, the catalytic activity is inhibited in the presence of ssDNA, as ssDNA can be adsorbed by MOFs and therefore protected the active sites from contact with substrates. Upon the addition of the T_DNA, double-stranded DNA(dsDNA) was formed and detached from the MOFs, resulting in the recovery of catalytic activity. Sequentially adding ssDNA or its complementary DNA strands can achieve the reversible regulation of the catalytic activity of MOFs nanozymes. Moreover, the DNA hybridization-based regulation was further applied to a cascaded catalytic system composed of the nanozyme, hemin@UiO-66-NH₂, and glucose oxidase. These nanozyme based programmable and reversibly regulated catalytic systems may have potential applications in future smart biosensing and catalysis systems.

Key words: Nanozyme, Catalytic metal-organic framework, Hemin, DNA hybridization, Dynamic modification

LIU Shuo, YANG Mingjie, GUO Weiwei. Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures[J]. 高等学校化学研究, 2020, 36(2): 301-306.

LIU Shuo, YANG Mingjie, GUO Weiwei. Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures[J]. Chemical Research in Chinese Universities, 2020, 36(2): 301-306.

参考文献 47

[1]	Gao L., Zhuang J., Nie L., Zhang J., Zhang Y., Gu N., Wang T., Feng J., Yang D., Perrett S., Yan X., Nat. Nanotechnol., 2007, 2, 577
[2]	Wei H., Wang E. K., Chem. Soc. Rev., 2013, 42, 6060
[3]	Huang Y., Ren J., Qu X., Chem. Rev., 2019, 119, 4357
[4]	Zhou H. C., Long J. R., Yaghi O. M., Chem. Rev., 2012, 112, 673
[5]	Chang Z., Yang D. H., Xu J., Hu T. L., Bu X. H., Adv. Mater., 2015, 27, 5432
[6]	Kitao T., Zhang Y., Kitagawa S., Wang B., Uemura T., Chem. Soc. Rev., 2017, 46, 3108
[7]	Yang S. J., Kim T., Im J. H., Kim Y. S., Lee K., Jung H., Park C. R., Chem. Mater., 2012, 24, 464
[8]	Alezi D., Belmabkhout Y., Suyetin M., Bhatt P. M., Weselinski L., Solovyeva V., Adil K., Spanopoulos I., Trikalitis I., Emwas A., Eddaoudi M., J. Am. Chem. Soc., 2015, 137, 13308
[9]	Bae T., Lee J. S., Qiu W., Koros W. J., Jones C. W., Nair S., Angew. Chem. Int. Ed., 2010, 122, 10059
[10]	Yang Q., Xu Q., Jiang H. L., Chem. Soc. Rev., 2017, 46, 4774
[11]	Dhakshinamoorthy A., Asiri A. M., Garcia H., Angew. Chem. Int. Ed., 2016, 55, 5414
[12]	Zhao M., Yuan K., Wang Y., Li G. D., Guo J., Gu L., Hu W. P., Zhao H. J., Tang Z. Y., Nature, 2016, 539, 76
[13]	Chen W. H., Yu X., Liao W. C., Sohn Y. S., Cecconello A., Kozell A. Nechushtai R., Willner I., Adv. Func. Mater., 2017, 27, 1702102
[14]	Wang Z., Fu Y., Kang Z., Liu X., Chen N., Wang Q., Tu Y., Wang L., Song S., Ling D., Song H., Kong X., Fan C., J. Am. Chem. Soc., 2017, 139, 15784
[15]	Duan Y., Ye F., Huang Y., Qin Y., He C., Zhao S., Chem. Commun., 2018, 54, 5377
[16]	Zheng M., Tan H., Xie Z., Zhang L., Jing X., Sun Z., ACS Appl. Mater. Interfaces, 2013, 5, 1078
[17]	Wu L. L., Wang Z., Zhao S. N., Meng X., Song X. Z., Feng J., Song S. Y., Zhang H. J., Chem. Eur. J., 2016, 22, 477
[18]	Lustig W. P., Mukherjee S., Rudd N. D., Desai A. V., Li J., Ghosh S. K., Chem. Soc. Rev., 2017, 46, 3242
[19]	Li S. L., Xu Q., Energ. Environ. Sci., 2013, 6, 1656
[20]	Zhang X., Li G., Wu D., Li X., Hu N., Chen J., Chen G., Wu Y., Biosens. Bioelectron., 2019, 137, 178
[21]	Li S., Liu X., Chai H., Huang Y., Trend. Anal. Chem., 2018, 105, 391
[22]	Lian X., Fang Y., Joseph E., Wang Q., Li J., Banerjee S., Lollar C., Wang X., Zhou H. C., Chem. Soc. Rev., 2017, 46, 3386
[23]	Qin F. X., Jia S. Y., Wang F. F., Wu S. H., Song J., Liu Y., Catal. Sci. Technol., 2013, 3, 276
[24]	Luo F., Lin Y., Zheng L., Lin X., Chi Y., ACS Appl. Mater. Interfaces, 2015, 7, 11322
[25]	Seeman N. C., Sleiman H. F., Nat. Rev. Mater., 2018, 3, 17068
[26]	Wang F., Lu C. H., Willner I., Chem. Rev., 2014, 114, 2881
[27]	Wang F., Liu X., Willner I., Angew. Chem. Int. Ed., 2015, 54, 1098
[28]	Zhou Y., Tang L., Zeng G., Zhang C., Zhang Y., Xie X., Sensor Actuat. B: Chem., 2016, 223, 280
[29]	Zhou L., Ren J., Qu X., Mater. Today, 2017, 20, 179
[30]	Kahn J. S., Freage L., Enkin N., Garcia M. A. A., Willner I., Adv. Mater., 2017, 29, 1602782
[31]	Zhu X., Zheng H. Y., Wei X. F., Lin Z. Y., Guo L. H., Qiu B., Chen G. N., Chem. Commun., 2013, 49, 127
[32]	Liu S., Wang L., Tian J. Q., Luo Y. L., Chang G. H., Abdullah A. M., Al-Youbi A. O., Sun X. P., ChemPlusChem, 2012, 77, 23
[33]	Zhang H., Zhang J., Huang G., Du Z., Jiang H., Chem. Commun., 2014, 50, 12069
[34]	Morris W., Briley W. E., Auyeung E., Cabezas M. D., Mirkin C. A., J. Am. Chem. Soc., 2014, 136, 7261
[35]	Chen W., Luo G., Sohn Y. S., Nechushtai R., Willner I., Adv. Funct. Mater., 2019, 29, 1805341
[36]	Cui L., Wu J., Li J., Ju H., Anal. Chem., 2015, 87, 10635
[37]	Zhang G., Shan D., Dong H., Cosnier S., Al-Ghanim K. A., Ahmad Z., Mahboob S., Zhang X., Anal. Chem., 2018, 90, 12284
[38]	Xie S., Ye J., Yuan Y., Chai Y., Yuan R., Nanoscale, 2015, 7, 18232
[39]	Wang C., Tang G., Tan H., Microchim. Acta, 2018, 185, 475
[40]	Tan B., Zhao H., Wu W., Liu X., Zhang Y., Quan X., Nanoscale, 2017, 9, 18699
[41]	Song C., Ding W., Liu H., Zhao W., Yao Y., Yao C., New J. Chem., 2019, 43, 12776
[42]	Luo F., Lin Y., Zheng L., Lin X., Chi Y., ACS Appl. Mater. Interfaces, 2015, 7, 11322
[43]	Alizadeh N., Salimi A., Hallaj R., Fathi F., Soleimani F., J. Nanobiotechnol., 2018, 16, 93
[44]	Wang Y., Wang J., Zhang Y., Li X., Fan M., Li M., J. Electrochem. Soc., 2018, 165, H673
[45]	Zhang L., Fan C., Liu M., Liu F., Bian S., Du S., Zhu S., Wang H., Sensor Actuat. B: Chem., 2018, 266, 543
[46]	Li D., Wu S., Wang F., Jia S., Liu Y., Han X., Zhang L., Zhang S., Wu Y., Mater. Lett., 2016, 178, 48
[47]	Zhang T., Wang L., Gao C., Zhao C., Wang Y., Wang J., Nanotechnology, 2018, 29, 074003

Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures

Programmable and Reversible Regulation of Catalytic Hemin@MOFs Activities with DNA Structures

可视化

摘要/Abstract

引用本文

使用本文

参考文献 47

相关文章 6

编辑推荐

Metrics

本文评价

[1]	HUA Cong, WANG Xuanzhong, LIANG Shipeng, LI Chen, CHEN Xi, PIAO Meihua, WANG Zhenchuan, GE Pengfei, LUO Tianfei. Maltol as a Novel Agent Protecting SH-SY5Y Cells Against Hemin-induced Ferroptosis[J]. 高等学校化学研究, 2022, 38(4): 1089-1096.
[2]	ZHU Junlun, CUI Qian, WEN Wei, ZHANG Xiuhua, WANG Shengfu. Cu/CuO-Graphene Foam with Laccase-like Activity for Identification of Phenolic Compounds and Detection of Epinephrine[J]. 高等学校化学研究, 2022, 38(4): 919-927.
[3]	LIU Lu, LIN Jingfang, SONG Yanling, YANG Chaoyong, ZHU Zhi. Effects of Molecular Crowding on G-Quadruplex-hemin Mediated Peroxidase Activity[J]. 高等学校化学研究, 2020, 36(2): 247-253.
[4]	XU Jia, ZHAO Xiaoming, YUAN Ye, SONG Yanhui, WANG Jiaqi, WANG Chonghan, CHEN Yujia, WANG Jianing, YAN Zhijun, GUAN Shuwen, WANG Liping. Screen, Design and Enzymatic Activity Determination of Artificial Microperoxidases[J]. 高等学校化学研究, 2018, 34(6): 934-938.
[5]	WANG Jiangning, SU Ping, LI Di, WANG Ting, YANG Yi. Fabrication of CeO₂/rGO Nanocomposites with Oxidase-like Activity and Their Application in Colorimetric Sensing of Ascorbic Acid[J]. 高等学校化学研究, 2017, 33(4): 540-545.
[6]	FENG Xi-zen, HOU Sun, CHAN Qi-lin, WANG Li-kai, QIN Ming, HAN Pei-dong . Microcontact Printing Techniques in Bioscience[J]. 高等学校化学研究, 2004, 20(6): 826-832.