Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions

doi:10.1007/s40242-021-1024-7

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 456-463.doi: 10.1007/s40242-021-1024-7

Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions

LI Haiyan¹, LIU Mingyue¹, WANG Xiaoyu¹, WANG Hongsheng¹, MO Xiumei¹, WU Jinglei^1,2

1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China;
2. Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P. R. China

收稿日期:2021-01-16 修回日期:2021-03-06 出版日期:2021-06-01 发布日期:2021-05-31
通讯作者: WU Jinglei E-mail:jw@dhu.edu.cn
基金资助:
This work was supported by the Fund of Donghua University, China (No.2232019D3-20), the Fund of the Science and Technology Commission of Shanghai Municipality, China(No.19440741300) and the National Natural Science Foundation of China(No.31900949).

Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions

LI Haiyan¹, LIU Mingyue¹, WANG Xiaoyu¹, WANG Hongsheng¹, MO Xiumei¹, WU Jinglei^1,2

1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China;
2. Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P. R. China

Received:2021-01-16 Revised:2021-03-06 Online:2021-06-01 Published:2021-05-31
Contact: WU Jinglei E-mail:jw@dhu.edu.cn
Supported by:
This work was supported by the Fund of Donghua University, China (No.2232019D3-20), the Fund of the Science and Technology Commission of Shanghai Municipality, China(No.19440741300) and the National Natural Science Foundation of China(No.31900949).

摘要/Abstract

摘要： Electrospun nanofibers are of the same length scale as the native extracellular matrix and have been extensively reported to facilitate adhesion and proliferation of cells and to promote tissue repair and regeneration. With a primary focus on tissue repair and regeneration using electrospun scaffolds, only a few studies involved electrospun nanofiber scaffolds directing cell behaviors have been reported. In this study, we prepared electrospun nanofiber scaffolds with distinct fiber configurations, namely, random and aligned orientations of nanofibers, as well as oriented yarns, and investigated their effects on cell behaviors. Our results showed that these scaffolds supported good proliferation and viability of murine fibroblasts. Fiber configuration profoundly influenced cell morpho-logy and orientation but showed no effects on cell proliferation rate. The yarn scaffold had comparable total protein accumulation with the random and aligned scaffolds, but it supported a greater pro-liferation rate of fibroblasts with significantly elevated collagen de-position due to its porous fibrous configuration. Cell-seeded yarn scaffolds showed a greater Young's modulus compared with cell-free controls as early as 1 week. Together with its unique fiber configuration similar to the native extracellular matrix of the myocardium, the yarn scaffold might be a suitable matrix material for modeling cardiac fibrotic disorders.

关键词: Electrospinning, Nanofiber configuration, Fibroblast, Cell proliferation, Biomechanics

Abstract: Electrospun nanofibers are of the same length scale as the native extracellular matrix and have been extensively reported to facilitate adhesion and proliferation of cells and to promote tissue repair and regeneration. With a primary focus on tissue repair and regeneration using electrospun scaffolds, only a few studies involved electrospun nanofiber scaffolds directing cell behaviors have been reported. In this study, we prepared electrospun nanofiber scaffolds with distinct fiber configurations, namely, random and aligned orientations of nanofibers, as well as oriented yarns, and investigated their effects on cell behaviors. Our results showed that these scaffolds supported good proliferation and viability of murine fibroblasts. Fiber configuration profoundly influenced cell morpho-logy and orientation but showed no effects on cell proliferation rate. The yarn scaffold had comparable total protein accumulation with the random and aligned scaffolds, but it supported a greater pro-liferation rate of fibroblasts with significantly elevated collagen de-position due to its porous fibrous configuration. Cell-seeded yarn scaffolds showed a greater Young's modulus compared with cell-free controls as early as 1 week. Together with its unique fiber configuration similar to the native extracellular matrix of the myocardium, the yarn scaffold might be a suitable matrix material for modeling cardiac fibrotic disorders.

Key words: Electrospinning, Nanofiber configuration, Fibroblast, Cell proliferation, Biomechanics

LI Haiyan, LIU Mingyue, WANG Xiaoyu, WANG Hongsheng, MO Xiumei, WU Jinglei. Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions[J]. 高等学校化学研究, 2021, 37(3): 456-463.

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Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions

Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions

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