pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

doi:10.1007/s40242-020-9067-8

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 285-290.doi: 10.1007/s40242-020-9067-8

pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

DONG Yuhang, PAN Xiaorui, LI Feng, YANG Dayong

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China

收稿日期:2019-11-27 修回日期:2019-12-23 出版日期:2020-04-01 发布日期:2020-03-18
通讯作者: LI Feng, YANG Dayong E-mail:dayong.yang@tju.edu.cn;fengli2018@tju.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.21621004, 21575101, 21622404, 21905196 and 31971305).

pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

DONG Yuhang, PAN Xiaorui, LI Feng, YANG Dayong

Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China

Received:2019-11-27 Revised:2019-12-23 Online:2020-04-01 Published:2020-03-18
Contact: LI Feng, YANG Dayong E-mail:dayong.yang@tju.edu.cn;fengli2018@tju.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21621004, 21575101, 21622404, 21905196 and 31971305).

摘要/Abstract

摘要： pH-Responsive DNA assembles have drawn growing attentions owing to their great potential in diverse areas. However, pH-responsive motifs are limited to specific DNA sequences and annealing is usually needed for DNA assemblies; therefore, sequence-independent pH-responsive DNA assembly at room temperature is highly desired as a more general way. Here, we propose a reversible pH-responsive DNA assembly strategy at room-temperature using zwitterion, glycine betaine(GB), as charge-regulation molecules. The reversible assembly and disassembly of DNA nanostructures could be achieved by alternatively regulating the acidic and basic environments in the presence of GB, respectively. In an acidic environment, carboxylate group in GB was protonated and GB was positively charged, which facilitated to shield the inherent electrostatic repulsion of DNA strands. Molecular simulation showed that the newly formed carboxyl group in protonated GB could form hydrogen bonds with bases in DNA to promote the assembly of DNA strands. In a basic solution, carboxylate group in GB was deprotonated and GB was neutral, thus inducing the dissociation of DNA assembly.

关键词: DNA nanotechnology, Dynamic assembly, pH responsiveness, Zwitterion

Abstract: pH-Responsive DNA assembles have drawn growing attentions owing to their great potential in diverse areas. However, pH-responsive motifs are limited to specific DNA sequences and annealing is usually needed for DNA assemblies; therefore, sequence-independent pH-responsive DNA assembly at room temperature is highly desired as a more general way. Here, we propose a reversible pH-responsive DNA assembly strategy at room-temperature using zwitterion, glycine betaine(GB), as charge-regulation molecules. The reversible assembly and disassembly of DNA nanostructures could be achieved by alternatively regulating the acidic and basic environments in the presence of GB, respectively. In an acidic environment, carboxylate group in GB was protonated and GB was positively charged, which facilitated to shield the inherent electrostatic repulsion of DNA strands. Molecular simulation showed that the newly formed carboxyl group in protonated GB could form hydrogen bonds with bases in DNA to promote the assembly of DNA strands. In a basic solution, carboxylate group in GB was deprotonated and GB was neutral, thus inducing the dissociation of DNA assembly.

Key words: DNA nanotechnology, Dynamic assembly, pH responsiveness, Zwitterion

DONG Yuhang, PAN Xiaorui, LI Feng, YANG Dayong. pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion[J]. 高等学校化学研究, 2020, 36(2): 285-290.

DONG Yuhang, PAN Xiaorui, LI Feng, YANG Dayong. pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion[J]. Chemical Research in Chinese Universities, 2020, 36(2): 285-290.

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pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

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