Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

doi:10.1007/s40242-018-7265-4

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (2): 229-234.doi: 10.1007/s40242-018-7265-4

Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

YU Yang^1,2, MA Wei³, YU Lina⁴, ZHOU Shuyuan⁵, ZHU Guangshan⁶, SHI Wei²

1. Department of Sports and Health, Guangzhou Institute of Physical Education, Guangzhou 510500, P. R. China;
2. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, College of Life Science, Jilin University, Changchun 130012, P. R. China;
3. China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China;
4. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, P. R. China;
5. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China;
6. Key Laboratory of Polyoxometalate Science, Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

收稿日期:2017-08-14 修回日期:2018-01-02 出版日期:2018-04-01 发布日期:2018-03-26
通讯作者: SHI Wei,E-mail:shiw@jlu.edu.cn E-mail:shiw@jlu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.51473060).

Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

YU Yang^1,2, MA Wei³, YU Lina⁴, ZHOU Shuyuan⁵, ZHU Guangshan⁶, SHI Wei²

1. Department of Sports and Health, Guangzhou Institute of Physical Education, Guangzhou 510500, P. R. China;
2. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, College of Life Science, Jilin University, Changchun 130012, P. R. China;
3. China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China;
4. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, P. R. China;
5. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China;
6. Key Laboratory of Polyoxometalate Science, Ministry of Education, Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

Received:2017-08-14 Revised:2018-01-02 Online:2018-04-01 Published:2018-03-26
Contact: SHI Wei,E-mail:shiw@jlu.edu.cn E-mail:shiw@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.51473060).

摘要/Abstract

摘要： In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average diameter was (109±9) nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate(FITC) was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was increased ≥ 10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.

关键词: Silica nanoparticle, Flow cytometry, Intracellular protein, Biomarker

Abstract: In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average diameter was (109±9) nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate(FITC) was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was increased ≥ 10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.

Key words: Silica nanoparticle, Flow cytometry, Intracellular protein, Biomarker

YU Yang, MA Wei, YU Lina, ZHOU Shuyuan, ZHU Guangshan, SHI Wei. Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro[J]. 高等学校化学研究, 2018, 34(2): 229-234.

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Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

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