Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

doi:10.1007/s40242-017-7240-5

高等学校化学研究 ›› 2017, Vol. 33 ›› Issue (6): 912-916.doi: 10.1007/s40242-017-7240-5

Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

WANG Zhongni^1,3, LIU Huijing¹, LI Luhua^1,3, LI Quanliang¹, WANG Xiuran¹, JIANG Yuan¹, FU Yuqin¹, LÜ Changli²

1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China;
3. Guizhou Sub-center of National Wheat Improvement Center, Guizhou University, Guiyang 550025, P. R. China

收稿日期:2017-07-14 修回日期:2017-10-10 出版日期:2017-12-01 发布日期:2017-10-16
通讯作者: FU Yuqin,E-mail:lucl055@nenu.edu.cn;LÜ Changli,E-mail:fuyuqin1974@163.com E-mail:lucl055@nenu.edu.cn;fuyuqin1974@163.com
基金资助:
Supported by the National Natural Science Foundation of China(Nos.31302062, 21074019) and the Youth Fund of Jilin Agricultural University, China(No.201315).

Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

WANG Zhongni^1,3, LIU Huijing¹, LI Luhua^1,3, LI Quanliang¹, WANG Xiuran¹, JIANG Yuan¹, FU Yuqin¹, LÜ Changli²

1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China;
3. Guizhou Sub-center of National Wheat Improvement Center, Guizhou University, Guiyang 550025, P. R. China

Received:2017-07-14 Revised:2017-10-10 Online:2017-12-01 Published:2017-10-16
Contact: FU Yuqin,E-mail:lucl055@nenu.edu.cn;LÜ Changli,E-mail:fuyuqin1974@163.com E-mail:lucl055@nenu.edu.cn;fuyuqin1974@163.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31302062, 21074019) and the Youth Fund of Jilin Agricultural University, China(No.201315).

摘要/Abstract

摘要： Although nanotechnology is considered to be one of the most important technologies to promote social and economic development in the twenty-first century, its application in agriculture is relatively few compared with those in other fields. In this article, plasmid carrying GUS gene was successfully delivered into tobacco(Nicotiana tabacum) by mesoporous silica nanoparticles(MSNs) modified with positively charged poly-L-lysine(PLL) with the assist of ultrasonic method. Stable transformation was confirmed by polymerase chain reaction(PCR) detection and GUS histochemical staining. Meanwhile, we also studied the factors that could enhance the genetic transformation efficiency. The result suggests that the callus receptor and a suitable DNA/MSNs ratio contribute a lot to the transformation efficiency. In a word, our research provides an efficient and cost-effective method for gene delivery to plant.

关键词: Mesoporous silica nanoparticle, Gene delivery, Tobacco

Abstract: Although nanotechnology is considered to be one of the most important technologies to promote social and economic development in the twenty-first century, its application in agriculture is relatively few compared with those in other fields. In this article, plasmid carrying GUS gene was successfully delivered into tobacco(Nicotiana tabacum) by mesoporous silica nanoparticles(MSNs) modified with positively charged poly-L-lysine(PLL) with the assist of ultrasonic method. Stable transformation was confirmed by polymerase chain reaction(PCR) detection and GUS histochemical staining. Meanwhile, we also studied the factors that could enhance the genetic transformation efficiency. The result suggests that the callus receptor and a suitable DNA/MSNs ratio contribute a lot to the transformation efficiency. In a word, our research provides an efficient and cost-effective method for gene delivery to plant.

Key words: Mesoporous silica nanoparticle, Gene delivery, Tobacco

WANG Zhongni, LIU Huijing, LI Luhua, LI Quanliang, WANG Xiuran, JIANG Yuan, FU Yuqin, LÜ Changli. Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco[J]. 高等学校化学研究, 2017, 33(6): 912-916.

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Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

Ultrasonic-assisted Mesoporous Silica Nanoparticle-mediated Exogenous Gene Stable Expression in Tobacco

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