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高等学校化学研究 ›› 2017, Vol. 33 ›› Issue (3): 507-512.doi: 10.1007/s40242-017-6473-7

• Articles • 上一篇    

Simultaneous Electrochemical Determination of Ascorbic Acid, Dopamine and Uric Acid Based on Reduced Graphene Oxide-Ag/PANI Modified Glassy Carbon Electrode

GUO Zhuo1, LUO Xianke1, LI Yahui2, LI Dongdi1, ZHAO Qinai1, LI Mengmeng1, MA Chi1, ZHAO Yanting1   

  1. 1. Department of Materials Science and Engineering, Shenyang University of Chemical Technology, Shenyang 110142, P. R. China;
    2. Shenzhen Graduate School of Harbin Institute of Technology, Shenzhen 518055, P. R. China
  • 收稿日期:2016-11-25 修回日期:2017-02-13 出版日期:2017-06-01 发布日期:2017-05-26
  • 通讯作者: GUO Zhuo,E-mail:guozhuochina@syuct.edu.cn E-mail:guozhuochina@syuct.edu.cn
  • 基金资助:

    Supported by the Natural Science Foundation of Liaoning Province, China(No.2015020197) and the National Natural Science Foundation of China(No.51403130).

Simultaneous Electrochemical Determination of Ascorbic Acid, Dopamine and Uric Acid Based on Reduced Graphene Oxide-Ag/PANI Modified Glassy Carbon Electrode

GUO Zhuo1, LUO Xianke1, LI Yahui2, LI Dongdi1, ZHAO Qinai1, LI Mengmeng1, MA Chi1, ZHAO Yanting1   

  1. 1. Department of Materials Science and Engineering, Shenyang University of Chemical Technology, Shenyang 110142, P. R. China;
    2. Shenzhen Graduate School of Harbin Institute of Technology, Shenzhen 518055, P. R. China
  • Received:2016-11-25 Revised:2017-02-13 Online:2017-06-01 Published:2017-05-26
  • Contact: GUO Zhuo,E-mail:guozhuochina@syuct.edu.cn E-mail:guozhuochina@syuct.edu.cn
  • Supported by:

    Supported by the Natural Science Foundation of Liaoning Province, China(No.2015020197) and the National Natural Science Foundation of China(No.51403130).

摘要:

An electrochemical method for simultaneous determination of ascorbic acid(AA), dopamine(DA) and uric acid(UA) was developed based on RGO-Ag/PANI modified glassy carbon electrode(RGO-Ag/PANI/GCE, RGO=reduced graphene oxide, PANI=polyaniline). Owing to the synergetic effect among RGO, Ag and PANI, the RGO-Ag/PANI based sensor not only improved the electrochemically catalytic oxidation of AA, DA and UA, but also resolved the overlapping anodic peaks. Under the optimized conditions, the calibration curves were obtained in the concentration ranges of 50-1000, 5-200 and 20-350 μmol/L, and the detection limits(S/N=3) of 0.5, 0.2 and 0.2 μmol/L were obtained for AA, DA, and UA, respectively, in neutral phosphate buffer solutions(PBS). The RGO-Ag/PANI/GCE sensor was successfully applied to the determination of AA, DA, and UA in bovine serum samples and showed high selectivity, sensitivity, and reproducibility.

关键词: Dopamine, Uric acid, Ascorbic acid, Electrochemical measurement

Abstract:

An electrochemical method for simultaneous determination of ascorbic acid(AA), dopamine(DA) and uric acid(UA) was developed based on RGO-Ag/PANI modified glassy carbon electrode(RGO-Ag/PANI/GCE, RGO=reduced graphene oxide, PANI=polyaniline). Owing to the synergetic effect among RGO, Ag and PANI, the RGO-Ag/PANI based sensor not only improved the electrochemically catalytic oxidation of AA, DA and UA, but also resolved the overlapping anodic peaks. Under the optimized conditions, the calibration curves were obtained in the concentration ranges of 50-1000, 5-200 and 20-350 μmol/L, and the detection limits(S/N=3) of 0.5, 0.2 and 0.2 μmol/L were obtained for AA, DA, and UA, respectively, in neutral phosphate buffer solutions(PBS). The RGO-Ag/PANI/GCE sensor was successfully applied to the determination of AA, DA, and UA in bovine serum samples and showed high selectivity, sensitivity, and reproducibility.

Key words: Dopamine, Uric acid, Ascorbic acid, Electrochemical measurement