Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

doi:10.1007/s40242-015-5088-0

高等学校化学研究 ›› 2015, Vol. 31 ›› Issue (6): 976-981.doi: 10.1007/s40242-015-5088-0

Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

FU Yuqin¹, LI Luhua¹, WANG Hui¹, JIANG Yuan¹, LIU Huijing¹, CUI Xiyan¹, WANG Piwu¹, LÜ Changli²

1. College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

收稿日期:2015-03-11 修回日期:2015-08-24 出版日期:2015-11-01 发布日期:2015-09-14
通讯作者: WANG Piwu, LÜ Changli E-mail:peiwuw@163.com;lucl055@nenu.edu.cn
基金资助:
Supported by the Science and Technology Development Project of Jilin Province, China(No.20090155), the National Natural Science Foundation of China(No.21574017) and the China Postdoctoral Science Foundation Funded Project(No.200904501024).

Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

FU Yuqin¹, LI Luhua¹, WANG Hui¹, JIANG Yuan¹, LIU Huijing¹, CUI Xiyan¹, WANG Piwu¹, LÜ Changli²

1. College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
2. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

Received:2015-03-11 Revised:2015-08-24 Online:2015-11-01 Published:2015-09-14
Contact: WANG Piwu, LÜ Changli E-mail:peiwuw@163.com;lucl055@nenu.edu.cn
Supported by:
Supported by the Science and Technology Development Project of Jilin Province, China(No.20090155), the National Natural Science Foundation of China(No.21574017) and the China Postdoctoral Science Foundation Funded Project(No.200904501024).

摘要/Abstract

摘要：

Nanoparticles as gene carriers become popular in the mammalian cells, whereas the application of them in plant cells is still very limited. Herein lies a report on silica nanoparticles(SiNPs) modified with positively charged poly-L-lysine(PLL) successfully delivering plasmid-encoded β-glucuronidase(GUS) gene into tobacco with the help of gene gun. The stable transgenic tobacco plants mediated by SiNPs can be obtained. Furthermore, we revealed the quantity of gene and types of receptor materials could affect the expression efficiency. In comparison to conventional gold particles-mediated transformation, the silica nanoparticles-mediated stable genetic transformation enhances transformation efficiency, potentially overcoming transgenic silencing. Our results demonstrate the great potential of SiNPs as gene carrier in plant genetic transformation and prove a novel approach for plant genetic decoration.

关键词: Silica nanoparticle, Gene carrier, Transformation, Stable expression

Abstract:

Key words: Silica nanoparticle, Gene carrier, Transformation, Stable expression

FU Yuqin, LI Luhua, WANG Hui, JIANG Yuan, LIU Huijing, CUI Xiyan, WANG Piwu, LÜ Changli. Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum[J]. 高等学校化学研究, 2015, 31(6): 976-981.

参考文献

[1] Sharma P., Brown S., Walter G., Santra S., Moudgil B., J. Colloid Interf. Sci., 2006, 123―126, 471
[2] Mintzer M. A., Simanek E. E., Chem. Rev., 2009, 109, 259
[3] Uchida M., Klem M. T., Allen M., Suci P., Flenniken M., Gillitzer E., Varpness Z., Liepold L. O., Young M., Douglas T., Adv. Mater., 2007, 19, 1025
[4] Marcel B. Jr., Moronne M., Gin P., Weiss S., Alivisatos A. P., Science, 1998, 281, 2013
[5] Xu Z. P., Zeng Q. H., Lu G. Q., Chem. Eng. Sci., 2006, 61, 1027
[6] Sokolova V., Epple M., Angew. Chem. Int. Ed., 2008, 47, 1382
[7] Nguyen D. N., Green J. J., Chan J. M., Langer R., Anderson D. G., Adv. Mater., 2009, 21, 847
[8] Wiesman Z., Dom N. B., Sharvit E., Grinberg S., Linder C., Heldman E., Zaccai M., J. Biotechnol., 2007, 130, 85
[9] Nair R., Varghese S. H., Nair B. G., Maekawa T., Yoshida Y., Kumar D. S., Plant Sci., 2010, 179, 154
[10] Torney F., Trewyn B. G., Lin V. S., Wang K., Nat. Nanotechnol., 2007, 2, 295
[11] Liu J., Wang F., Wang L., Xiao S., Tong C., Tang D., Liu X., J. Cent. South Univ. T., 2008, 15, 768
[12] Pasupathy K., Lin S., Hu Q., Luo H., Ke P. C., Biotechnol. J., 2008, 3, 1078
[13] Song Y., Li Y., Cui H. X., Li Y., Biotechnol. Bull., 2009, 6, 75
[14] Köping-Höggård M., Tubulekas I., Guan H., Edwards K., Nilsson M., Vårum K., Artursson P., Gene Ther., 2001, 8, 1108
[15] Bharali D. J., Klejbor I., Stachowiak E. K., Dutta P., Roy I., Kaur N., Bergey E. J., Prasad P. N., Stachowiak M. K., Proc. Natl. Acad. Sci.USA, 2005, 102, 11539
[16] Trewyn B. G., Giri S., Slowing I. I., Lin V. S., Chem. Commun., 2007, 31, 3236
[17] Hom C., Lu J., Liong M., Luo H., Li Z., Zink J. I., Tamanoi F., Small, 2010, 6, 1185
[18] Barnes C. A., Elsaesser A., Arkusz J., Smok A., Palus J., Le?niak A., Salvati A., Hanrahan J. P., Jong W. H., Dziuba?towska E., Nano. Lett., 2008, 8, 3069
[19] Boussif O., Lezoualc'h F., Zanta M. A., Mergny M. D., Scherman D., Demeneix B., Behr J. P., Proc. Natl. Acad. Sci.USA, 1995, 92, 7297
[20] Bivas-Benita M., Romeijn S., Junginger H. E., Borchard G., Eur. J. Pharm. Biopharm., 2004, 58, 1
[21] He X., Nie H., Wang K., Tan W., Wu X., Zhang P., Anal. Chem., 2008, 80, 9597
[22] Burns A. A., Vider J., Ow H., Herz E., Penate-Medina O., Baumgart M., Larson S. M., Wiesner U., Bradbury M., Nano Lett., 2008, 9, 442
[23] Fu Y. Q., Li L. H., Wang P. W., Qu J., Fu Y. P., Wang H., Sun J. R., Lu C. L., Chem. Res. Chinese Universities, 2012, 28(4), 672
[24] Stöber W., Fink A., Bohn E., J. Colloid Interface Sci., 1968, 26, 62
[25] Jefferson R. A., Plant Mol. Bio. Rep., 1987, 5, 387
[26] McCabe M. S., Power J. B., de Laat A. M., Davey M. R., Mol Biotechnol., 1997, 7, 79
[27] Yu H.Y., Tang Z. H., Song W. H., Deng M. X., Chen X. S., Chem. J. Chinese Universities, 2014, 35(5), 903
[28] Xiu Y., Zhou Y., Gao Q., Chen J. S., Li G. D., Chem. Res. Chinese Universities, 2013, 29(1),189
[29] Periyasamy S. V., Othalathara U. A., Bashir M. K., Bhagavatula L. V. P., Adv. Funct. Mater., 2010, 20, 2416
[30] Susana M. O., Justin S. V., Victor S. Y. L., Brian G. T., Kan W., Adv. Funct. Mater., 2012, 22, 3576

Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

Silica Nanoparticles-mediated Stable Genetic Transformation in Nicotiana tabacum

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