Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (5): 912-915 .

Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

WANG Xiao-qing¹, CHEN Qi-hui¹, HOU Yu-chuan¹, LU Zhi-hua¹, HU Jing-hai^,, ZHANG Hai-feng¹, HAO Yuan-yuan¹, ZHANG Long¹, GAO Zhan-tuan², WANG Chun-xi^1*

1. Department of Urology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022, P. R. China

收稿日期:2011-12-07 修回日期:2012-02-13 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: WANG Chun-xi E-mail:chunxi_wang@126.com
基金资助:
Supported by the National Natural Science Foundation of China(No. 50973043).

Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

WANG Xiao-qing¹, CHEN Qi-hui¹, HOU Yu-chuan¹, LU Zhi-hua¹, HU Jing-hai^,, ZHANG Hai-feng¹, HAO Yuan-yuan¹, ZHANG Long¹, GAO Zhan-tuan², WANG Chun-xi^1*

1. Department of Urology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2011-12-07 Revised:2012-02-13 Online:2012-09-25 Published:2012-09-07
Supported by:
Supported by the National Natural Science Foundation of China(No. 50973043).

摘要/Abstract

摘要： A degradable poly(lactic-co-glycolic acid, LA:GA=80:20)(PLGA) urethral tubular scaffold was fabricated by electrospinning. In order to enhance the mechanical properties, the scaffold was crosslinked with glutaraldehyde. The structure and properties of the crosslinked scaffolds were investigated by the mechanical property testing, scanning electron microscopy(SEM), degradability test in vitro and 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-diphenytetrazo- liumromide(MTT). The results show that the scaffold has the nano-structure. The pore size and the porosity are suitable for cell seeding, growth and extracellular matrix production. Although influenced by the crosslinking slightly, the pore size and the porosity could still support cell proliferation and tissuse formation. The mechanical properties are remarkably increased by the crosslinking of glutaraldehyde, and it could meet the demands of a urethral stent. The scaffold could completely collapse within 70 d. The results of the biocompatibility test show that the PLGA scaffold had no cytotoxicity.

关键词: Poly(lactic-co-glycolic acid), Urethral scaffold, Degradable, Electrospin, Crosslinking

Abstract: A degradable poly(lactic-co-glycolic acid, LA:GA=80:20)(PLGA) urethral tubular scaffold was fabricated by electrospinning. In order to enhance the mechanical properties, the scaffold was crosslinked with glutaraldehyde. The structure and properties of the crosslinked scaffolds were investigated by the mechanical property testing, scanning electron microscopy(SEM), degradability test in vitro and 3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-diphenytetrazo- liumromide(MTT). The results show that the scaffold has the nano-structure. The pore size and the porosity are suitable for cell seeding, growth and extracellular matrix production. Although influenced by the crosslinking slightly, the pore size and the porosity could still support cell proliferation and tissuse formation. The mechanical properties are remarkably increased by the crosslinking of glutaraldehyde, and it could meet the demands of a urethral stent. The scaffold could completely collapse within 70 d. The results of the biocompatibility test show that the PLGA scaffold had no cytotoxicity.

Key words: Poly(lactic-co-glycolic acid), Urethral scaffold, Degradable, Electrospin, Crosslinking

WANG Xiao-qing, CHEN Qi-hui, HOU Yu-chuan, LU Zhi-hua, HU Jing-hai, ZHANG Hai-feng, HAO Yuan-yuan, ZHANG Long, GAO Zhan-tuan, WANG Chun-xi. Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold[J]. 高等学校化学研究, 2012, 28(5): 912-915 .

[1]	SUN Bolun, CHAO Danming, WANG Ce. Piezoelectric Nanogenerator Based on Electrospun Cellulose Acetate/Nanocellulose Crystal Composite Membranes for Energy Harvesting Application[J]. 高等学校化学研究, 2022, 38(4): 1005-1011.
[2]	YANG Siran, AI Feixue, LI Ziping, ZHAO Guiyan, BI Yanfeng. N-Doped Carbon Nanofibers Encapsulating CoO@Co₉S₈ Nanoparticles: Preparation from S-Rich Co₃₂ Coordination Cluster Precursors by Electrospinning and Application for Superior Li-ion Storage[J]. 高等学校化学研究, 2022, 38(2): 603-608.
[3]	Matthew J. OWEN, Jasper H. N. YIK, Congwang YE, Brianca NETTO, Dominik R. HAUDENSCHILD, Gang-yu LIU. A Green Approach to Producing Polymer Microparticles for Local Sustained Release of Flavopiridol[J]. 高等学校化学研究, 2021, 37(5): 1116-1124.
[4]	LI Haiyan, LIU Mingyue, WANG Xiaoyu, WANG Hongsheng, MO Xiumei, WU Jinglei. Nanofiber Configuration of Electrospun Scaffolds Dictating Cell Behaviors and Cell-scaffold Interactions[J]. 高等学校化学研究, 2021, 37(3): 456-463.
[5]	HONG Huoyan, ZHANG Dongdong, LIN Si, HAN Feng, WANG Kaili, JIANG Di, WU Jinglei, MO Xiumei, WANG Hongsheng. Green Electrospun Silk Fibroin Nanofibers Loaded with Cationic Ethosomes for Transdermal Drug Delivery[J]. 高等学校化学研究, 2021, 37(3): 488-495.
[6]	ZHENG Gaofeng, PENG Hao, JIANG Jiaxin, KANG Guoyi, LIU Juan, ZHENG Jianyi, LIU Yifang. Surface Functionalization of PEO Nanofibers Using a TiO₂ Suspension as Sheath Fluid in a Modified Coaxial Electrospinning Process[J]. 高等学校化学研究, 2021, 37(3): 571-577.
[7]	ZHANG Xiuling, GUO Shiquan, QIN Yue, LI Congju. Functional Electrospun Nanocomposites for Efficient Oxygen Reduction Reaction[J]. 高等学校化学研究, 2021, 37(3): 379-393.
[8]	CHEN Zhijun, HAO Ming, QIAN Xiaoming, CHEN Wenyang, ZENG Ming, HUANG Juan, LI Ruixin, FAN Jintu, LIU Yanbo. Characterization on Modification and Biocompatibility of PCL Scaffold Prepared with Near-field Direct-writing Melt Electrospinning[J]. 高等学校化学研究, 2021, 37(3): 578-583.
[9]	WANG Jun, FU Wanlin, XU Wanlin, WU Min, SUN Yueming, DAI Yunqian. Oxide Nanofibers as Catalysts Toward Energy Conversion and Environmental Protection[J]. 高等学校化学研究, 2021, 37(3): 366-378.
[10]	HUANG Hedong, GUO Zeyu, YANG Pengyan, CHEN Peng, Wu Jie. Electrical Conductivity, Oil Absorption and Electric Heating of Carbon Black-modified Carbon Nanofibers[J]. 高等学校化学研究, 2021, 37(3): 541-548.
[11]	BAI Shan, ZHANG Xiangyu, ZANG Leilei, YANG Songze, CHEN Xiaoqi, YUAN Xiaoyan. Electrospinning of Biomaterials for Vascular Regeneration[J]. 高等学校化学研究, 2021, 37(3): 394-403.
[12]	QIN Mei, LIU Daqing, DAI Zhang, MENG Xin, LIU Guosai, LIU Hao, HUANG Xiaowei, YAN Xu, CHEN Shaojuan. One Step Fabrication and Application of Antibacterial Electrospun Zein/Cinnamon Oil Membrane Wound Dressing via In situ Electrospinning Process[J]. 高等学校化学研究, 2021, 37(3): 464-469.
[13]	XIANG Huilin, ZHONG Lingling, REN Yongqing, LIU Dongmei, ZHU Zhigao, XU Ying, WANG Yu, WANG Wei. Superhydrophobized Polyacrylonitrile/Hierarchicall-FeOOH Nanofibrous Membrane for High-salinity Water Treatment in Membrane Distillation[J]. 高等学校化学研究, 2021, 37(3): 470-479.
[14]	CAO Wenhao, WANG Caifeng, WANG Shuai, ZHANG Yang, ZHAO Ruisheng. Preparation of Photoresponsive PAN-NH₂@EPESP Fiber Films with Mechanical Stability for Regulating Wettability and Micro-environment Humidity[J]. 高等学校化学研究, 2021, 37(3): 512-521.
[15]	FENG Zhendong, ZHANG Xiaopei, LIU Na, WANG Yue, ZHOU Ziyi, GLEBOV O. Oleg, WU Tong. Promotion of Neurite Outgrowth and Extension Using Injectable Welded Nanofibers[J]. 高等学校化学研究, 2021, 37(3): 522-527.

Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

Fabrication, Crosslinking and in vitro Biocompatibility of a Novel Degradable Nano-structure Urethral Tubular Scaffold

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价