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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (4): 604-608.

• Articles • 上一篇    下一篇

Determination of Biotin in Pharmaceutical Formulations by Potassium Permanganate-luminol-CdTe Nanoparticles Chemiluminescence System

TRAORE Zoumana Sékou, SU Xing-guang   

  1. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • 收稿日期:2011-10-14 修回日期:2012-03-10 出版日期:2012-07-25 发布日期:2012-07-25
  • 通讯作者: SU Xing-guang E-mail:suxg@jlu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.21075050) and the Science and Technology Development Project of Jilin Province, China(No.20110334).

Determination of Biotin in Pharmaceutical Formulations by Potassium Permanganate-luminol-CdTe Nanoparticles Chemiluminescence System

TRAORE Zoumana Sékou, SU Xing-guang   

  1. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • Received:2011-10-14 Revised:2012-03-10 Online:2012-07-25 Published:2012-07-25
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.21075050) and the Science and Technology Development Project of Jilin Province, China(No.20110334).

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被引次数

10

摘要： A sensitive flow-injection chemiluminescence method was developed for the determination of biotin in the pharmaceutical formulations. The affinity between avidin and biotin was used to adsorb biotin on the polystyrene, with subsequent quantification of biotin based on its ability to enhance the chemiluminescence(CL) signal generated by the redox reaction of potassium permanganate-luminol-CdTe nanoparticles CL system. The investigations prove that apart from 3-aminophthalate, the CdTe quantum dots(QDs) play both catalytic and emitter roles. Under optimum conditions, the linear range for the determination of biotin was 0.01-25ng/mL with a detection limit of 7.3?10-3 ng/mL(S/N=3). The relative standard deviation of 5 ng/L biotin was 2.06%(n=7). The proposed method was used to determine the biotin concentration in the pharmaceutical formulations and the recovery was between 96.4% and 104%. The proposed method is simple, convenient, rapid and sensitive.

关键词: Flow-injection, Chemiluminescence, Biotin, CdTe nanoparticle

Abstract: A sensitive flow-injection chemiluminescence method was developed for the determination of biotin in the pharmaceutical formulations. The affinity between avidin and biotin was used to adsorb biotin on the polystyrene, with subsequent quantification of biotin based on its ability to enhance the chemiluminescence(CL) signal generated by the redox reaction of potassium permanganate-luminol-CdTe nanoparticles CL system. The investigations prove that apart from 3-aminophthalate, the CdTe quantum dots(QDs) play both catalytic and emitter roles. Under optimum conditions, the linear range for the determination of biotin was 0.01-25ng/mL with a detection limit of 7.3?10-3 ng/mL(S/N=3). The relative standard deviation of 5 ng/L biotin was 2.06%(n=7). The proposed method was used to determine the biotin concentration in the pharmaceutical formulations and the recovery was between 96.4% and 104%. The proposed method is simple, convenient, rapid and sensitive.

Key words: Flow-injection, Chemiluminescence, Biotin, CdTe nanoparticle