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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (4): 672-676.

• Articles • 上一篇    下一篇

Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles

吕长利   

  1. 1. College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
    2. Faculty of Agronomy, Jilin Agricultural University, Changchun 130018, P. R. China;
    3. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China
  • 收稿日期:2011-10-24 修回日期:2012-12-23 出版日期:2012-07-25 发布日期:2012-07-25
  • 通讯作者: WANG Pi-wu, L? Chang-li E-mail:peiwuw@yahoo.com.cn;lucl055@nenu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.21074019), the China Postdoctoral Science Foundation Funded Project(No.200904501024), the Natural Science Foundation of Jilin Province, China(No.20101539) and the Jilin Province Science and Technology Development Project, China(No.20090155).

Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles

changli lv   

  1. 1. College of Life Science, Jilin Agricultural University, Changchun 130018, P. R. China;
    2. Faculty of Agronomy, Jilin Agricultural University, Changchun 130018, P. R. China;
    3. Faculty of Chemistry, Northeast Normal University, Changchun 130024, P. R. China
  • Received:2011-10-24 Revised:2012-12-23 Online:2012-07-25 Published:2012-07-25
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.21074019), the China Postdoctoral Science Foundation Funded Project(No.200904501024), the Natural Science Foundation of Jilin Province, China(No.20101539) and the Jilin Province Science and Technology Development Project, China(No.20090155).

PDF (PC)

被引次数

19

摘要： The development of nanotechnology provides a new method for genetic engineering. However, the nanoparticles as gene carriers have been mainly used in the mammalian cells so far. We observed that ZnS nanoparticles modified with positively charged poly-L-lysine(PLL) successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted method. Polymerase chain reaction(PCR) detection, Southern blot analysis and GUS histochemical staining were carried out for the regenerated plants. The stable genetic modified plants mediated by ZnS nanoparticles can be obtained. This article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.

关键词: ZnS nanoparticle, Gene carrier, Transformation, Tobacco, Stable expression

Abstract: The development of nanotechnology provides a new method for genetic engineering. However, the nanoparticles as gene carriers have been mainly used in the mammalian cells so far. We observed that ZnS nanoparticles modified with positively charged poly-L-lysine(PLL) successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted method. Polymerase chain reaction(PCR) detection, Southern blot analysis and GUS histochemical staining were carried out for the regenerated plants. The stable genetic modified plants mediated by ZnS nanoparticles can be obtained. This article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.

Key words: ZnS nanoparticle, Gene carrier, Transformation, Tobacco, Stable expression