[an error occurred while processing this directive]

高等学校化学研究 ›› 1998, Vol. 14 ›› Issue (4): 420-422.

• Articles • 上一篇    下一篇

A Simple Method Designed for the Separation of Biotinylated Ligate from Free Biotin

LI Tian-yu, ZHOU Chang-cheng, FANG Rui, QI Jie, ZHOU Hui, LI Wei   

  1. Department of Life Science, Jilin University, Changchun 130023
  • 收稿日期:1997-09-17 出版日期:1998-12-24 发布日期:2011-08-17
  • 通讯作者: LI Wei

A Simple Method Designed for the Separation of Biotinylated Ligate from Free Biotin

LI Tian-yu, ZHOU Chang-cheng, FANG Rui, QI Jie, ZHOU Hui, LI Wei   

  1. Department of Life Science, Jilin University, Changchun 130023
  • Received:1997-09-17 Online:1998-12-24 Published:2011-08-17

摘要: Considering the chemical characteristics of the biotinylate reagent,we designed a method for the separation of biotinylatedlig ate from residual biot inylate reagent with equal efficiency to ultr afiltration.This metho disespecially fit for the separation of biotinylating small molecules.

关键词: Biotinylated Ligate, Biotin, Protein

Abstract: Considering the chemical characteristics of the biotinylate reagent,we designed a method for the separation of biotinylatedlig ate from residual biot inylate reagent with equal efficiency to ultr afiltration.This metho disespecially fit for the separation of biotinylating small molecules.

Key words: Biotinylated Ligate, Biotin, Protein