高等学校化学研究 ›› 1998, Vol. 14 ›› Issue (4): 414-419.
Hydrophobic Pocket Modification and Humanized Catalytic Antibodies with Glutathione Peroxidase Activity(Ⅰ)——Preparation and Characterization of Haptenes and Conjugates
WANG Lin1, MA Xue-yan1, DING Lan1, ZHAO Da-qing1, NI Jia-zuan1, FU Ping-ping2, LI Xiao-ping3, JIN Xiang-jun3
- 1. Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022;
2. Norman Bethune University of Medicine Sciences, Changchun 130021;
3. Department of Chemistry, Baicheng Normal College, Baicheng 137000
Hydrophobic Pocket Modification and Humanized Catalytic Antibodies with Glutathione Peroxidase Activity(Ⅰ)——Preparation and Characterization of Haptenes and Conjugates
WANG Lin1, MA Xue-yan1, DING Lan1, ZHAO Da-qing1, NI Jia-zuan1, FU Ping-ping2, LI Xiao-ping3, JIN Xiang-jun3
- 1. Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022;
2. Norman Bethune University of Medicine Sciences, Changchun 130021;
3. Department of Chemistry, Baicheng Normal College, Baicheng 137000
摘要： The thiol group of glutathione(GSH)was protected by 2,4-dinitrochlorobenzene(DNCB),the product S-substituted dinitrophenyl GSH(GSH-S-DNP)was alcoholized to obta in haptenes 4 and 5 respectively.As haptenes,the two GSHderivatives were characterized by means of 1 HNMR,MALDI-T OF-MS and IR,followed by individually coupling with bovine serumalbumin(BSA)viag lutar aldehyde.BSA-Hp4 and BSA-Hp5 were purified by Sephadex G-25 gel filtration chromatography.For each conjugate,the average haptene-BSA ratio was 12:1.The electro phoresis analysis showed that the average molecular weight of each conjugate was 76 500.The CD spectrum indicated that the conjugates had more-helix content than BSA did.