Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (2): 236-238.

Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

CUI Wei, HUANG Lei, LI Wei, WANG Li-ping

College of Life Science, Jilin University, Changchun 130021, P.R.China

收稿日期:2006-01-10 出版日期:2006-04-24 发布日期:2011-08-06

Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

CUI Wei, HUANG Lei, LI Wei, WANG Li-ping

College of Life Science, Jilin University, Changchun 130021, P.R.China

Received:2006-01-10 Online:2006-04-24 Published:2011-08-06

摘要/Abstract

摘要： The S-adenosylmethionine synthetase (SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids and involved in a number of biochemical reactions.In this study, a cDNA containing the coding sequence for rat liver SAM synthetase was cloned into the prokaryotic expression vector pQE30 and expressed in E.coli M15.A major band corresponding to a protein of 48 kDa was detected on SDS-PAGE.The protein was distributed in both the soluble fraction and the insoluble fraction.In soluble fractions the protein was fully active.

关键词: SAM, SAM synthetase, Cloning, Expression, HPLC

Abstract: The S-adenosylmethionine synthetase (SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids and involved in a number of biochemical reactions.In this study, a cDNA containing the coding sequence for rat liver SAM synthetase was cloned into the prokaryotic expression vector pQE30 and expressed in E.coli M15.A major band corresponding to a protein of 48 kDa was detected on SDS-PAGE.The protein was distributed in both the soluble fraction and the insoluble fraction.In soluble fractions the protein was fully active.

Key words: SAM, SAM synthetase, Cloning, Expression, HPLC

CUI Wei, HUANG Lei, LI Wei, WANG Li-ping. Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase[J]. 高等学校化学研究, 2006, 22(2): 236-238.

CUI Wei, HUANG Lei, LI Wei, WANG Li-ping. Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase[J]. Chemical Research in Chinese Universities, 2006, 22(2): 236-238.

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Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase

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