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高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (2): 197-200.

• Articles • 上一篇    下一篇

A Repressor in 5'-UTR of hKv4.3 Gene

LI Hao1,2, JIANG Chun-lai1, YU Xiang-hui1, WU Yong-ge1, LI Wei1, KONG Wei1   

  1. 1. College of Life Science, Jilin University, Changchun 130012, P.R.China;
    2. Jilin Teacher's Institute of Engineering Technology, Changchun 130052, P.R.China
  • 收稿日期:2005-12-31 出版日期:2006-04-24 发布日期:2011-08-06
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.30170215).

A Repressor in 5'-UTR of hKv4.3 Gene

LI Hao1,2, JIANG Chun-lai1, YU Xiang-hui1, WU Yong-ge1, LI Wei1, KONG Wei1   

  1. 1. College of Life Science, Jilin University, Changchun 130012, P.R.China;
    2. Jilin Teacher's Institute of Engineering Technology, Changchun 130052, P.R.China
  • Received:2005-12-31 Online:2006-04-24 Published:2011-08-06
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30170215).

PDF (PC)

摘要： The effect of repressors on ion channel gene expression was studied.The hKv4.3 promoter and the sequence (+ 2-+ 160, S160) of 5'-UTR of the hKv4.3 gene was cloned into the pβ-gal-Basic vector.The transient expression of the pβ-gal vector and the analysis of the relative activity of β-galactosidase were carried out.The analysis of the mRNA level was carried out with the RT-PCR method.S160 could intensively repress the expression of the hKv4.3 gene with position-specificity.The level of mRNA did not alter obviously.A repressor(S160), in 5'-UTR of the hKv4.3 gene was found and its repression to gene expression may play a role in the post-transcription process.

关键词: hKv4.3 gene, Repressor

Abstract: The effect of repressors on ion channel gene expression was studied.The hKv4.3 promoter and the sequence (+ 2-+ 160, S160) of 5'-UTR of the hKv4.3 gene was cloned into the pβ-gal-Basic vector.The transient expression of the pβ-gal vector and the analysis of the relative activity of β-galactosidase were carried out.The analysis of the mRNA level was carried out with the RT-PCR method.S160 could intensively repress the expression of the hKv4.3 gene with position-specificity.The level of mRNA did not alter obviously.A repressor(S160), in 5'-UTR of the hKv4.3 gene was found and its repression to gene expression may play a role in the post-transcription process.

Key words: hKv4.3 gene, Repressor