Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (3): 319-323.

Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

LI Zi-jian¹, LIU Ning², LIU Zhi-qiang², LIU Shu-ying², HAN Qi-de¹, ZHANG You-yi¹

1. Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022, P. R. China

收稿日期:2005-09-07 出版日期:2006-06-24 发布日期:2011-08-06
基金资助:
Supported by the National Key Basic Research Program of China(No. G2000056906), the National Natural Science Foundation of China(Nos. 30400552 and 20273067) and the Research Fund for the Doctoral Program of Higher Education(No. 20030001014).

Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

LI Zi-jian¹, LIU Ning², LIU Zhi-qiang², LIU Shu-ying², HAN Qi-de¹, ZHANG You-yi¹

1. Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, P. R. China;
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022, P. R. China

Received:2005-09-07 Online:2006-06-24 Published:2011-08-06
Supported by:
Supported by the National Key Basic Research Program of China(No. G2000056906), the National Natural Science Foundation of China(Nos. 30400552 and 20273067) and the Research Fund for the Doctoral Program of Higher Education(No. 20030001014).

摘要/Abstract

摘要： β-Adrenoceptors(β-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through whichβ-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol(ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 ± 70spots were detected and about 1191 e 54 spots were matched, with an average matching rate of 92. 9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS.

关键词: Proteome, Two-dimensional electrophoresis(2-DE), MALDI-MS, &beta, -Adrenoceptor, Cardiomyocyte

Abstract: β-Adrenoceptors(β-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through whichβ-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol(ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 ± 70spots were detected and about 1191 e 54 spots were matched, with an average matching rate of 92. 9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS.

Key words: Proteome, Two-dimensional electrophoresis(2-DE), MALDI-MS, β-Adrenoceptor, Cardiomyocyte

LI Zi-jian, LIU Ning, LIU Zhi-qiang, LIU Shu-ying, HAN Qi-de, ZHANG You-yi. Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation[J]. 高等学校化学研究, 2006, 22(3): 319-323.

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Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

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