Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (4): 550-556.

Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

ZHANG Xia^1*, LIU Wei¹, XU Yong¹, YANG Liu², KONG Wei-song¹, RUI Xiao-dong¹, YANG Shuai¹, CHEN Yong-kuan¹ and MIAO Ming-ming¹

1. Key Laboratory of Tobacco Chemistry, Yunnan Academy of Tobacco Science, Kunming 650106, P. R. China;
2. Research and Development Center of Hongta Tobacco(Group) Corporation Limited, Yuxi 653100, P. R. China

收稿日期:2011-02-13 修回日期:2011-06-20 出版日期:2011-07-25 发布日期:2011-06-29
通讯作者: ZHANG Xia E-mail:zhangxia840511@163.com
基金资助:
Supported by the Foundation of State Tabacco Monopoly Bureau(China) in Research Project(No.110200902008).

Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

ZHANG Xia^1*, LIU Wei¹, XU Yong¹, YANG Liu², KONG Wei-song¹, RUI Xiao-dong¹, YANG Shuai¹, CHEN Yong-kuan¹ and MIAO Ming-ming¹

1. Key Laboratory of Tobacco Chemistry, Yunnan Academy of Tobacco Science, Kunming 650106, P. R. China;
2. Research and Development Center of Hongta Tobacco(Group) Corporation Limited, Yuxi 653100, P. R. China

Received:2011-02-13 Revised:2011-06-20 Online:2011-07-25 Published:2011-06-29
Contact: ZHANG Xia E-mail:zhangxia840511@163.com
Supported by:
Supported by the Foundation of State Tabacco Monopoly Bureau(China) in Research Project(No.110200902008).

摘要/Abstract

摘要： The comparison of solid phase extraction(SPE) for the preconcentration and isolation of polyphenols in tobacco samples was carried out by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and multivariate analysis. Several adsorbing materials of SPE(C18, NH2, SAX and OASIS) were investigated. It was found that the C18 and OASIS cartridges can not only speed up the purification process, but also simplify the SPE operation. A UPLC/MS/MS was used for the determination of polyphenols in tobacco samples after purification. All analytes were separated and determined in 2 min. The limit of detection was 0.05 ng/mL. Cluster analysis(CA) and principal component analysis(PCA) were used for the analysis of 4 varieties(flue-cured tobacco, oriental tobacco, sun-cured tobacco and burley) in order to interpret the effect of planting and machining process on the concentration of polyphenols. The different types of tobacco samples could be easily clustered by CA. PCA on the chemical composition of tobacco resulted in two principal components(PCs) that take 84.2% of the total variance. The PCA and CA indicate that the polyphenols can be used for distinguishing tobacco types.

关键词: Solid phase extraction, Polyphenol, Ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC/MS/MS), Tobacco, Cluster analysis, Principal component analysis

Abstract: The comparison of solid phase extraction(SPE) for the preconcentration and isolation of polyphenols in tobacco samples was carried out by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and multivariate analysis. Several adsorbing materials of SPE(C18, NH2, SAX and OASIS) were investigated. It was found that the C18 and OASIS cartridges can not only speed up the purification process, but also simplify the SPE operation. A UPLC/MS/MS was used for the determination of polyphenols in tobacco samples after purification. All analytes were separated and determined in 2 min. The limit of detection was 0.05 ng/mL. Cluster analysis(CA) and principal component analysis(PCA) were used for the analysis of 4 varieties(flue-cured tobacco, oriental tobacco, sun-cured tobacco and burley) in order to interpret the effect of planting and machining process on the concentration of polyphenols. The different types of tobacco samples could be easily clustered by CA. PCA on the chemical composition of tobacco resulted in two principal components(PCs) that take 84.2% of the total variance. The PCA and CA indicate that the polyphenols can be used for distinguishing tobacco types.

Key words: Solid phase extraction, Polyphenol, Ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC/MS/MS), Tobacco, Cluster analysis, Principal component analysis

ZHANG Xia*, LIU Wei, XU Yong, YANG Liu, KONG Wei-song, RUI Xiao-dong, .... Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis[J]. 高等学校化学研究, 2011, 27(4): 550-556.

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Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

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