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高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (6): 929-933.

• Articles • 上一篇    下一篇

Flow-injection Spectrophotometric Determination of Uric Acid in Urine via Prussian Blue Reaction

WASEEM Amir1*, YAQOOB Mohammad2, NABI Abdul2, MURTAZA Ghulam3 and HUSSAIN Izhar3   

  1. 1. Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan;
    2. Department of Chemistry, University of Balochistan, Quetta 87300, Pakistan;
    3. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
  • 收稿日期:2011-01-19 修回日期:2011-04-29 出版日期:2011-11-25 发布日期:2011-11-07
  • 通讯作者: WASEEM Amir E-mail:waseemq2000@hotmail.com
  • 基金资助:

    Supported by the CIIT-Project Funded by COMSATS Institute of Information Technology, Pakistan.

Flow-injection Spectrophotometric Determination of Uric Acid in Urine via Prussian Blue Reaction

WASEEM Amir1*, YAQOOB Mohammad2, NABI Abdul2, MURTAZA Ghulam3 and HUSSAIN Izhar3   

  1. 1. Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan;
    2. Department of Chemistry, University of Balochistan, Quetta 87300, Pakistan;
    3. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan
  • Received:2011-01-19 Revised:2011-04-29 Online:2011-11-25 Published:2011-11-07
  • Contact: WASEEM Amir E-mail:waseemq2000@hotmail.com
  • Supported by:

    Supported by the CIIT-Project Funded by COMSATS Institute of Information Technology, Pakistan.

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被引次数

3

摘要： A simple and sensitive flow injection(FI) spectrophotometric method was reported for the determination of uric acid based on the reduction of Fe(III)/ferricyanide in the presence of uric acid. The in situ reduced ions reacted with unreduced portion of ferricyanide/Fe(III) to form soluble Prussian blue, which was monitored at an absorption wavelength of 735 nm. The optimized conditions allow a linear calibration graph in a concentration range of 1―100 μmol/L. The relative standard deviation was in a range of 0.5%―2.5%, with a detection limit(3σ blank) of 0.3 μmol/L and a sampling frequency of 60 injection/h was obtained. The effect of common substances present in human physiological fluids on the determination of uric acid was examined. The method was applied to determining uric acid in human urine samples with the recoveries in a range of 96%―105%. The results agree well with those by spectrophotometric reference method at a confidence level of 95%. Spectrophotometric procedures for uric acid determination in clinical samples were reviewed briefly.

关键词: Flow injection analysis, Spectrophotometry, Uric acid, Prussian blue, Urine

Abstract: A simple and sensitive flow injection(FI) spectrophotometric method was reported for the determination of uric acid based on the reduction of Fe(III)/ferricyanide in the presence of uric acid. The in situ reduced ions reacted with unreduced portion of ferricyanide/Fe(III) to form soluble Prussian blue, which was monitored at an absorption wavelength of 735 nm. The optimized conditions allow a linear calibration graph in a concentration range of 1―100 μmol/L. The relative standard deviation was in a range of 0.5%―2.5%, with a detection limit(3σ blank) of 0.3 μmol/L and a sampling frequency of 60 injection/h was obtained. The effect of common substances present in human physiological fluids on the determination of uric acid was examined. The method was applied to determining uric acid in human urine samples with the recoveries in a range of 96%―105%. The results agree well with those by spectrophotometric reference method at a confidence level of 95%. Spectrophotometric procedures for uric acid determination in clinical samples were reviewed briefly.

Key words: Flow injection analysis, Spectrophotometry, Uric acid, Prussian blue, Urine