Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus

doi:10.1007/s40242-025-5008-x

高等学校化学研究 ›› 2025, Vol. 41 ›› Issue (2): 358-366.doi: 10.1007/s40242-025-5008-x

• Articles • 上一篇

Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus

DONG Jiaxin¹, SU Rui², WU Xiaokang¹, DONG Deming¹, WANG Yining¹, HUANG Chenni¹, QIU Tao¹, GU Yu¹, HUA Xiuyi¹, LIANG Dapeng¹

1. Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130012, P. R. China;
2. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China

收稿日期:2025-01-10 接受日期:2025-03-05 出版日期:2025-04-01 发布日期:2025-03-31
通讯作者: SU Rui,rsu@jlu.edu.cn;LIANG Dapeng,liangdp@jlu.edu.cn E-mail:rsu@jlu.edu.cn;liangdp@jlu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (No. 22376084).

Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus

DONG Jiaxin¹, SU Rui², WU Xiaokang¹, DONG Deming¹, WANG Yining¹, HUANG Chenni¹, QIU Tao¹, GU Yu¹, HUA Xiuyi¹, LIANG Dapeng¹

1. Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun 130012, P. R. China;
2. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2025-01-10 Accepted:2025-03-05 Online:2025-04-01 Published:2025-03-31
Contact: SU Rui,rsu@jlu.edu.cn;LIANG Dapeng,liangdp@jlu.edu.cn E-mail:rsu@jlu.edu.cn;liangdp@jlu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No. 22376084).

摘要/Abstract

摘要： The study of the metabolic toxicity of perfluorooctanoic acid (PFOA) is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment, and provides a fundamental scientific basis for environmental protection and sustainable development. In this study, an electrospray ionization (ESI) source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid (PFOA) and their correlations in skin mucus and liver tissue of tilapia. Seven common metabolites were found in mucus and liver, and nitrogen metabolism pathways were disturbed in all of them. The upregulation of glutamate in mucus may reflect an increased demand for amino acids and energy, whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism, protein synthesis and amino acid metabolism. Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways, which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.

关键词: Perfluorooctanoic acid (PFOA), Electrospray ionization-mass spectrometry (ESI-MS), Fish skin mucus, Nitrogen metabolism

Abstract: The study of the metabolic toxicity of perfluorooctanoic acid (PFOA) is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment, and provides a fundamental scientific basis for environmental protection and sustainable development. In this study, an electrospray ionization (ESI) source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid (PFOA) and their correlations in skin mucus and liver tissue of tilapia. Seven common metabolites were found in mucus and liver, and nitrogen metabolism pathways were disturbed in all of them. The upregulation of glutamate in mucus may reflect an increased demand for amino acids and energy, whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism, protein synthesis and amino acid metabolism. Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways, which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.

Key words: Perfluorooctanoic acid (PFOA), Electrospray ionization-mass spectrometry (ESI-MS), Fish skin mucus, Nitrogen metabolism

DONG Jiaxin, SU Rui, WU Xiaokang, DONG Deming, WANG Yining, HUANG Chenni, QIU Tao, GU Yu, HUA Xiuyi, LIANG Dapeng. Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus[J]. 高等学校化学研究, 2025, 41(2): 358-366.

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Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus

Probing PFOA-induced Metabolic Disorders in Tilapia Through Fish Skin Mucus

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