NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form

doi:10.1007/s40242-021-1022-9

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 795-800.doi: 10.1007/s40242-021-1022-9

NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form

HAO Xueyu^1,2, LI Chunjie¹, WANG Yu^1,2, ZHANG Feng¹, HOU Jingwei¹, KANG Chunqing^1,2, GAO Lianxun¹

1. Laboratory of Polymer Composite and Engineering, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. University of Science and Technology of China, Hefei 230026, P. R. China

收稿日期:2021-01-15 修回日期:2021-02-09 出版日期:2021-06-01 发布日期:2021-03-10
通讯作者: KANG Chuanqing E-mail:kangcq@ciac.ac.cn
基金资助:
The authors appreciate the technical help of Mr. LI Haoyu(Changchun Institute of Applied Chemistry, Chinese Academy of Sciences) for insightful comments and suggestions.

NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form

HAO Xueyu^1,2, LI Chunjie¹, WANG Yu^1,2, ZHANG Feng¹, HOU Jingwei¹, KANG Chunqing^1,2, GAO Lianxun¹

1. Laboratory of Polymer Composite and Engineering, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. University of Science and Technology of China, Hefei 230026, P. R. China

Received:2021-01-15 Revised:2021-02-09 Online:2021-06-01 Published:2021-03-10
Contact: KANG Chuanqing E-mail:kangcq@ciac.ac.cn
Supported by:
The authors appreciate the technical help of Mr. LI Haoyu(Changchun Institute of Applied Chemistry, Chinese Academy of Sciences) for insightful comments and suggestions.

摘要/Abstract

摘要： G-Quadruplexes(GQs), which are formed by G-rich DNA sequences in human telomeres, have become an attractive target for cancer treatment. The ligands to stabilize the conformation of human telomeric GQs in vivo are particularly important for structure-based ligand design and drug development targeting the noncanonical DNA structure. Here we report the conformational conversion of Tel26 induced by a naphthalene diimide(NDI) ligand in K⁺ buffer, even at cellular physiological temperature(37℃) and under mimetic cellular crowding conditions created by Ficoll 70. We provide an insight into the dynamic conversion from initial hybrid-2 GQ topology to final parallel GQ topology. These results are helpful for the design of ligands with GQ conformation regulation.

关键词: G-Quadruplex, Naphthalene diimide, Topological conversion, Telomeric DNA

Abstract: G-Quadruplexes(GQs), which are formed by G-rich DNA sequences in human telomeres, have become an attractive target for cancer treatment. The ligands to stabilize the conformation of human telomeric GQs in vivo are particularly important for structure-based ligand design and drug development targeting the noncanonical DNA structure. Here we report the conformational conversion of Tel26 induced by a naphthalene diimide(NDI) ligand in K⁺ buffer, even at cellular physiological temperature(37℃) and under mimetic cellular crowding conditions created by Ficoll 70. We provide an insight into the dynamic conversion from initial hybrid-2 GQ topology to final parallel GQ topology. These results are helpful for the design of ligands with GQ conformation regulation.

Key words: G-Quadruplex, Naphthalene diimide, Topological conversion, Telomeric DNA

HAO Xueyu, LI Chunjie, WANG Yu, ZHANG Feng, HOU Jingwei, KANG Chunqing, GAO Lianxun. NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form[J]. 高等学校化学研究, 2021, 37(3): 795-800.

参考文献 31

[1]	Williamson J. R., Raghuraman M. K., Cech T. R., Cell, 1989, 59(5), 871
[2]	Jerry W. S., Woodring E. W., Nat. Rev. Genet., 2019, 20(5), 299
[3]	Zhou X., Xing D., Chem. Soc. Rev., 2012, 41(13), 4643
[4]	Ruggiero E., Richter S. N., Nucleic Acids Res., 2018, 46(7), 3270
[5]	Ahmed A. A., Marchetti C., Ohnmacht S. A., Stephen N., Sci. Rep., 2020, 10(1), 12192
[6]	Ahmed A. A., Angell R., Oxenford S., Worthington J., Williams N., Barton N., Fowler T. G., OFlynn D. E., Sunose M., McConvile M., Vo T., Wilson W. D., Karim S. A., Morton J. P., Neidle S., ACS Med. Chem. Lett., 2020, 11(8), 1634
[7]	Zhang Z., Dai J., Veliath E., Jones R.A., Yang D., Nucleic Acids Res., 2010, 38(3), 1009
[8]	Dai J., Carver M., Punchihewa C., Jones R. A., Yang D., Nucleic Acids Res., 2007, 35(15), 4927
[9]	Lin C., Wu G., Wang K., Onel B., Sakai S., Shao Y., Yang D., Angew. Chem. Int. Ed., 2018, 57(34), 10888
[10]	Liu W., Zhong Y., Liu L., Shen C., Zeng W., Wang F., Yang D., Mao Z., Nat. Commun., 2018, 9, 3496
[11]	Barthwal R., Raje S., Pandav K., Bioorg. Med. Chem., 2020, 28(23), 115761
[12]	Yu Q., Wang M., Bioorg. Med. Chem., 2020, 28(17), 115641
[13]	Wang Z., Li M., Chen W., Hsu S., Chang T., Nucleic Acids Res., 2016, 44(8), 3958
[14]	Xu L., Feng S., Zhou X., Chem Commun., 2011, 47(12), 3517
[15]	Xue Y., Kan Z. Y., Wang Q., Yao Y., Liu J., Hao Y. H., Tan Z., J. Am. Chem. Soc., 2007, 129(36), 11185
[16]	Heddi B., Phan A. T., J. Am. Chem. Soc., 2011, 133(25), 9824
[17]	Xue Y., Liu J., Zheng K., Kan Z., Hao Y., Tan Z., Angew. Chem. Int. Ed., 2011, 50(35), 8046
[18]	Hänsel R., Löhr F., Foldynová-Trantirková S., Bamberg E., Trantírek L., Dötsch V., Nucleic Acids Res., 2011, 39(13), 5768
[19]	Zuffo M., Guédin A., Leriche E., Doria F., Pirota V., Gabelica V., Mergny J., Freccero M., Nucleic Acids Res., 2018, 46(19), e115
[20]	Hao X., Wang C., Wang Y., Li C., Hou J., Zhang F., Kang Q., Gao L., Int. J. Biol. Macromol., 2021, 167, 1048
[21]	Takahashi S., Yamamoto J., Kitamura A., Kinjo M., Sugimoto N., Anal. Chem., 2019, 91(4), 2586
[22]	Vo T., Oxenford S., Angell R., Marchetti C., Ohnmacht S. A., Wilson W. D., Neidle S., ACS Med. Chem. Lett., 2020, 11(5), 991
[23]	Parkinson G. N., Cuenca F., Neidle S., J. Mol. Biol., 2008, 381(5), 1145
[24]	Mpima S., A Ohnmacht S. A., Barletta M., Husby J., Pett L. C., Gunaratnam M., Hilton S. T., Neidle S., Bioorg. Med. Chem., 2013, 21(20), 6162
[25]	Guo Y., Chen J., Cheng M., Monchaud D., Zhou J., Ju H., Angew. Chem. Int. Ed., 2017, 56(52), 16636
[26]	Wu J., Meng Z., Lu Y., Shao F., Chem. Eur. J., 2017, 23(56), 13980
[27]	Mashimo T., Yagi H., Sannohe Y., Rajendran A., Sugiyama H., J. Am. Chem. Soc., 2010, 132(42), 14910
[28]	Li W., Hou X., Wang P., Xi X., Li M., J. Am. Chem. Soc., 2013, 135(17), 6423
[29]	Marchand A., Gabeliaca V., Nucleic Acids Res., 2016, 44(22), 10999
[30]	Maleki P., Budhathoki J. B., Roy W. A., Balci H., Mol. Gen. Genomics, 2017, 292(3), 483
[31]	Hou X., Fu Y., Wu W., Wang L., Teng F., Xie P., Wang P., Xi X., Nucleic Acids Res., 2017, 45(19), 11401

NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form

NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form

可视化

摘要/Abstract

引用本文

使用本文

参考文献 31

相关文章 5

编辑推荐

Metrics

本文评价

[1]	LIU Lu, LIN Jingfang, SONG Yanling, YANG Chaoyong, ZHU Zhi. Effects of Molecular Crowding on G-Quadruplex-hemin Mediated Peroxidase Activity[J]. 高等学校化学研究, 2020, 36(2): 247-253.
[2]	ZHU Yuqing, LI Wei, TAN Suzhen, CHEN Tianxiao. A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex Molecular Beacon for the Detection of Kanamycin[J]. 高等学校化学研究, 2018, 34(4): 541-545.
[3]	ZHANG Hong, ZOU Li, LI Ruimin, ZHAO Mingqin, LING Liansheng. Hairpin Probe for Sequence-specific Recognition of Double-stranded DNA on Simian Virus 40[J]. 高等学校化学研究, 2018, 34(1): 28-32.
[4]	QI Fuling, GAO Lianxun, HAN Fushe. Design and Synthesis of Macrocyclic Polyoxazoles[J]. 高等学校化学研究, 2014, 30(4): 587-592.
[5]	Jeremy Green, Li ming Ying, David Klenerman, Shankar Blasubramanian. Single-Molecule FRET Study of DNA G-Quadruplex[J]. 高等学校化学研究, 2002, 18(2): 103-106.