Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea

doi:10.1007/s40242-018-7335-7

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (4): 691-696.doi: 10.1007/s40242-018-7335-7

• Articles • 上一篇

Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea

CHEN Xiaoqiang^1,2,3, SHAO Shengrong^1,4, XIE Jianchun², YUAN Hao⁵, LI Qian¹, WU Long¹, WU Zhengqi¹, YUAN Haibo³, JIANG Yongwen³

1. College of Bioengineering and Food, Hubei University of Technology, Wuhan 430068, P. R. China;
2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, P. R. China;
3. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, P. R. China;
4. Shaoxing Royal Tea Village Co., Ltd., Shaoxing 312037, P. R. China;
5. Wuhan Wugang Fast Food & Beverage Co., Ltd., Wuhan 430068, P. R. China

收稿日期:2017-10-24 出版日期:2018-08-01 发布日期:2018-06-19
通讯作者: CHEN Xiaoqiang, YUAN Haibo E-mail:biomed528@163.com;192168092@tricaas.com
基金资助:
Supported by the Chutian Scholar Program of Hubei Province, China and the Fund of Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, China(No.20161012).

Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea

CHEN Xiaoqiang^1,2,3, SHAO Shengrong^1,4, XIE Jianchun², YUAN Hao⁵, LI Qian¹, WU Long¹, WU Zhengqi¹, YUAN Haibo³, JIANG Yongwen³

1. College of Bioengineering and Food, Hubei University of Technology, Wuhan 430068, P. R. China;
2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, P. R. China;
3. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, P. R. China;
4. Shaoxing Royal Tea Village Co., Ltd., Shaoxing 312037, P. R. China;
5. Wuhan Wugang Fast Food & Beverage Co., Ltd., Wuhan 430068, P. R. China

Received:2017-10-24 Online:2018-08-01 Published:2018-06-19
Contact: CHEN Xiaoqiang, YUAN Haibo E-mail:biomed528@163.com;192168092@tricaas.com
Supported by:
Supported by the Chutian Scholar Program of Hubei Province, China and the Fund of Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, China(No.20161012).

摘要/Abstract

摘要： Tea polysaccharide conjugates(TPCs) were water-extracted and purified from low-grade green tea. Although TPC contains 1.97% covalent binding proteins, Coomassie brilliant blue G250 and R250 could not detect this protein. The "should-shape" absorption peak observed at 250-280 nm in the UV-Vis spectrum was caused by quinone compounds derived from polyphenol oxidation during TPC extraction. The UV-Vis spectrum could be used to detect the decolorizing degree of TPC. TPC aqueous solution merely presented the negative charge properties of its polysaccharides instead of the acid-base property of its protein section, and exhibited higher stability at pH greater than 5.0. No precipitation or haze occurred in three TPC/epigallocatechin gallate(EGCG) aqueous mixtures during 12 h storage. TPC had emulsifying activity, which indicated that its protein moiety formed hydrophobic groups. It is proposed that some of the physicochemical properties of the TPC protein moiety are shielded by its polysaccharide chain.

关键词: Green tea, Polysaccharide conjugate, Protein

Abstract: Tea polysaccharide conjugates(TPCs) were water-extracted and purified from low-grade green tea. Although TPC contains 1.97% covalent binding proteins, Coomassie brilliant blue G250 and R250 could not detect this protein. The "should-shape" absorption peak observed at 250-280 nm in the UV-Vis spectrum was caused by quinone compounds derived from polyphenol oxidation during TPC extraction. The UV-Vis spectrum could be used to detect the decolorizing degree of TPC. TPC aqueous solution merely presented the negative charge properties of its polysaccharides instead of the acid-base property of its protein section, and exhibited higher stability at pH greater than 5.0. No precipitation or haze occurred in three TPC/epigallocatechin gallate(EGCG) aqueous mixtures during 12 h storage. TPC had emulsifying activity, which indicated that its protein moiety formed hydrophobic groups. It is proposed that some of the physicochemical properties of the TPC protein moiety are shielded by its polysaccharide chain.

Key words: Green tea, Polysaccharide conjugate, Protein

CHEN Xiaoqiang, SHAO Shengrong, XIE Jianchun, YUAN Hao, LI Qian, WU Long, WU Zhengqi, YUAN Haibo, JIANG Yongwen. Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea[J]. 高等学校化学研究, 2018, 34(4): 691-696.

参考文献

[1] Nie S. P., Xie M. Y., Food Hydrocolloids, 2011, 25(2), 144
[2] Chen H., Wang Z., Qu Z., Europe Food Research Technology, 2009, 229(4), 629
[3] Chen X., Lin Z., Ye Y., Zhang R., Yin J., Jiang Y., Wan H., Carbohydrate Polymers, 2010, 82(1), 28
[4] Wang D., Li J., Wang C., Zhao G., Jin Y., Cheng D., Ye S., Journal of Tea Science, 2000, 20, 45
[5] Fu B. Q., Xie M. Y., Zhou P., Nie S., Wang Y., Journal of Wuxi University of Light Industry, 2002, 21(4), 362
[6] Huang Y. C., Ma Y. F., Xie Q. R., Huang X. Y., Huang Y. N., Food Science, 2007, 28, 170
[7] Lu X. S., Zhao Y., Sun Y. F., Yang S., Yang X., Food Chemistry, 2013, 141(4), 3415
[8] Chen H. X., Qu Z. S., Fu L. L., Dong P., Zhang X., Journal of Food Science, 2009, 74(6),C469
[9] Chen X., Ye Y., Cheng H., Jiang Y., Wu Y., Agric. Food Chem., 2009, 57(13), 5795
[10] Zhang C., Xiao L., Biochemistry Experiment, Chemical Industry Press, Beijing, 2009
[11] Bradford M., Biochem., 1976, 72(1), 248
[12] Sambrook J. F., Russell D. W., Molecular Cloning:A Laboratory Manual(3rd Ed.), Cold Spring Harbor Laboratory Press, New York, 2001
[13] Chen X., Fang Y., Nishinari K., We H., Sun C., Li J., Jiang Y., Carbohyd. Polym., 2014, 112(21), 77
[14] Al-Assaf S., Phillips G., LC GC N. Am., 2008, 26(2), 196
[15] Li X., Fang Y., Al-Assaf S., Phillips G., Jiang F., Colloid Interface Sci., 2012, 388(1), 103
[16] Chen X. Q., Zhang Z. F., Gao Z. M., Huang Y., Wu Z. Q., Interna-tional Journal of Biological Macromolecules, 2017, 104, 1294
[17] Chen X. Q., Song W., Zhao J., Molecules, 2017, 22(6), 914
[18] Wang J., Zhu S., Xu C., Biochemistry(3rd Ed.), Higher Education Press, Beijing, 2002
[19] Shimizu M. M., Melo G. A., Brombini D. S. A., Plant Physiology Biochemistry, 2011, 49(9), 970
[20] Mishra B. B., Gautam S., Sharma A., Postharvest Biology & Tech-nology, 2012, 67(7), 44
[21] Wang M., Hu Z., Zhou M., Chinese Bulletin of Botany, 2005, 22(2), 215
[22] Haslam E., Phytochemistry, 2003, 64(1), 61
[23] Tanaka T., Kouno I., Food Science & Technology Research, 2003, 9(2), 128
[24] Matthew E. H., Douglas A. B., Alan P. D., Critical Reviews in Plant Sciences, 1997, 16(5), 415
[25] Sang S., Yang I., Buckley B., Free Radical Biology & Medicine, 2007, 43(3), 362
[26] Sang S., Pharmacological Research, 2011, 64(2), 87
[27] Ha C., Natural Products Chemistry and Applications(2nd Ed.), Chemical Industry Press, Beijing, 2003, 333
[28] Ozawa T., Lilley T., Haslam E., Phytochemistry, 1987, 26(11), 2937
[29] Luck G., Liao H., Murray N., Phytochemistry, 1994, 37(2), 357
[30] Haslam E., Nat. Prod., 1996, 59(2), 205
[31] Dickinson E., Food Hydrocoll, 2009, 23(6), 1473
[32] Li X. D., Fu Y., Ma L. N.,Wang Z. X., Zhang H. M., Chem. J. Chinese Universities, 2016, 32(3), 343

Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea

Analysis of Protein Moiety of Polysaccharide Conjugates Water-extracted from Low Grade Green Tea

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	HU Lingling, LIU Ke, REN Guolan, LIANG Jiangong, WU Yuan. Progress in DNA Aptamers as Recognition Components for Protein Functional Regulation[J]. 高等学校化学研究, 2022, 38(4): 894-901.
[2]	ZHANG Mengqian, HE Peiyang, LI Yanmei. Contemporary Approaches to α,β-Dehydroamino Acid Chemical Modifications[J]. 高等学校化学研究, 2021, 37(5): 1044-1054.
[3]	TANG Lin, ZENG Xiaodong, ZHOU Hui, GUI Conghao, LUO Qiulin, ZHOU Wenyi, WU Jing, LI Qianqian, LI Yang, XIAO Yuling. Theranostic Gold Nanoclusters for NIR-II Imaging and Photodynamic Therapy[J]. 高等学校化学研究, 2021, 37(4): 934-942.
[4]	ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei. Chemical Synthesis of Proteins Containing 300 Amino Acids[J]. 高等学校化学研究, 2020, 36(5): 733-747.
[5]	LI Xue, YANG Donglei, SHEN Luyao, XU Fan, WANG Pengfei. Programmable Assembly of DNA-protein Hybrid Structures[J]. 高等学校化学研究, 2020, 36(2): 211-218.
[6]	WANG Ruijue, SHENG Kai, HOU Yingqin, SUN Jialing, LU Hua. Tailoring Cationic Helical Polypeptides for Efficient Cytosolic Protein Delivery[J]. 高等学校化学研究, 2020, 36(1): 134-138.
[7]	ZHOU Tong, XU Guangyu, SUN Luyao, YU Zhenxiang, WANG Guixia. Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice[J]. 高等学校化学研究, 2019, 35(3): 427-433.
[8]	LI Xinhua, YUAN Caixia, LU Liping, ZHU Miaoli, XING Shu, FU Xueqi. Exploration of Zinc(II) Complexes as Potent Inhibitors Against Protein Tyrosine Phosphatase 1B[J]. 高等学校化学研究, 2019, 35(2): 186-192.
[9]	CHEN Weizhu, ZHANG Yiping, SUN Jipeng, XIE Quanling, HONG Zhuan, YI Ruizao. Rapid Determination of Tetrodotoxin in Human Plasma by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. 高等学校化学研究, 2018, 34(6): 905-911.
[10]	YUE Ling. QM/MM Investigations on the Bioluminescent Decomposition of Coelenterazine Dioxetanone in Obelin[J]. 高等学校化学研究, 2018, 34(5): 758-766.
[11]	YU Yang, MA Wei, YU Lina, ZHOU Shuyuan, ZHU Guangshan, SHI Wei. Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro[J]. 高等学校化学研究, 2018, 34(2): 229-234.
[12]	WANG Shihui, SONG Yuming, WANG Yue, GAO Yang, YU Shanshan, ZHAO Qianqian, JIN Xiangqun, LU Haibin. Studies on New Steroidal Saponins from Allii macrostemonis Bulbus and Their Antitumor Activities Design and Synthesis of Novel Bispecific Molecules for InducingBRD4 Protein Degradation[J]. 高等学校化学研究, 2018, 34(1): 67-74.
[13]	WEI Yaozhu, TAN Xiangshi. Insights into the Interactions Between Corrinoid Iron-sulfur Protein and Methyl Transferase from Human Pathogen Clostridium difficile[J]. 高等学校化学研究, 2017, 33(5): 731-735.
[14]	PAN Dong, WANG Lincong, LIU Meiyi, JIN Shi, WANG Liyan, YU Xianghui, ZHA Xiao, WU Yuqing. Effects of Site-directed Mutagenesis of L469 in Helix-5 of Human Papillomavirus 16 L1 on Pentamer Formation[J]. 高等学校化学研究, 2017, 33(3): 392-399.
[15]	HOU Xuemei, ZHAO Changjie, TIAN Yanlong, DOU Shuliang, ZHANG Xiang, ZHAO Jiupeng. Preparation of Functionalized Fe₃O₄@SiO₂ Magnetic Nanoparticles for Monoclonal Antibody Purification[J]. 高等学校化学研究, 2016, 32(6): 889-894.