高等学校化学研究 ›› 2017, Vol. 33 ›› Issue (3): 339-342.doi: 10.1007/s40242-017-7051-8
Therapeutic Drug Monitoring of Vancomycin and Voriconazole by Liquid Chromatography-Tandem Mass Spectrometric Method
LI Yanyan1, YIN Lei1,2, LI Yanhua1, SUN Zhihui1, ZHAO Xiaojun1, GAO Mingyue1, WANG Hongliang1
- 1. The First Hospital of Jilin University, Changchun 130021, P. R. China;
2. School of Life Science, Jilin University, Changchun 130012, P. R. China
Therapeutic Drug Monitoring of Vancomycin and Voriconazole by Liquid Chromatography-Tandem Mass Spectrometric Method
LI Yanyan1, YIN Lei1,2, LI Yanhua1, SUN Zhihui1, ZHAO Xiaojun1, GAO Mingyue1, WANG Hongliang1
- 1. The First Hospital of Jilin University, Changchun 130021, P. R. China;
2. School of Life Science, Jilin University, Changchun 130012, P. R. China
摘要：
A reliable liquid chromatography-tandem mass spectrometric(LC-MS/MS) method was developed and validated for the simultaneous determination of vancomycin and voriconazole in human plasma. The analytes and internal standard 10-hydroxycarbazepine were separated at a flow rate of 0.9 mL/min using a Zorbax SB-C18 column (50 mm×4.6 mm, 2.7 μm). Positive ion electrospray ionization was used to detect vancomycin, voriconazole and intrenal standard(IS) 10-hydroxycarbazepine followed by multiple reaction monitoring(MRM) of the transition at m/z 725.5→144.2, 350.3→281.0 and 253.1→208.0, respectively. The total run time for both vancomycin and voriconazole samples was 5 min; 0.30 μg/mL was the lower limit of quantification. The precision of intraday and interday was no more than 12.4%. The method was successfully and resoundingly applied in therapeutic drug monitoring of 156 patients treated with vancomycin and voriconazole.