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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (6): 1026-1030.

• Articles • 上一篇    下一篇

Hepato-protective Effect of Polysaccharides Extracted from Inula Britannica Flower for Mice

HONG Tie1, DONG Man1, GAO Yan2, ZHAO Jing1, WU Yi1   

  1. 1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
    2. College of Pharmacy, Zhengzhou University, Zhengzhou 450001, P. R. China
  • 收稿日期:2012-06-14 修回日期:2012-07-06 出版日期:2012-11-25 发布日期:2012-11-09
  • 通讯作者: HONG Tie,WU Yi E-mail:hongtie@jlu.edu.cn;wuyi@jlu.edu.cn
  • 基金资助:

    Supported by the Science and Technology Development Program of Jilin Province, China(No.20070917-01) and the Technology Development Project of Jilin Province, China(No.201205020).

Hepato-protective Effect of Polysaccharides Extracted from Inula Britannica Flower for Mice

HONG Tie1, DONG Man1, GAO Yan2, ZHAO Jing1, WU Yi1   

  1. 1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
    2. College of Pharmacy, Zhengzhou University, Zhengzhou 450001, P. R. China
  • Received:2012-06-14 Revised:2012-07-06 Online:2012-11-25 Published:2012-11-09
  • Contact: HONG Tie,WU Yi E-mail:hongtie@jlu.edu.cn;wuyi@jlu.edu.cn
  • Supported by:

    Supported by the Science and Technology Development Program of Jilin Province, China(No.20070917-01) and the Technology Development Project of Jilin Province, China(No.201205020).

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摘要：

The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity. The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography(HPLC) and infrared spectroscopy(IR) methods. The results show that the molecular weight distribution of IBP is from 600 to 3500. The weight-average molecular weight is 1099. IBP consists of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose and arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9. The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins. After administration of IBP with 100, 50 and 25 mg/kg body mass to liver injury mice induced by CCl4 or bacillus calmette guerin-lipopolysaccharide (BCG-LPS), not only the blood alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels decreased significantly(P<0.05), but aslo the liver tissue malondialdehyde(MDA) level decreased significantly(P<0.01). The superoxide dismutase(SOD) level increased significantly(P<0.01). IBP shows significant anti-liver injury and anti- oxidation activity.

关键词: Inula britannica flower, Polysaccharide, Anti-liver injury

Abstract:

The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity. The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography(HPLC) and infrared spectroscopy(IR) methods. The results show that the molecular weight distribution of IBP is from 600 to 3500. The weight-average molecular weight is 1099. IBP consists of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose and arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9. The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins. After administration of IBP with 100, 50 and 25 mg/kg body mass to liver injury mice induced by CCl4 or bacillus calmette guerin-lipopolysaccharide (BCG-LPS), not only the blood alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels decreased significantly(P<0.05), but aslo the liver tissue malondialdehyde(MDA) level decreased significantly(P<0.01). The superoxide dismutase(SOD) level increased significantly(P<0.01). IBP shows significant anti-liver injury and anti- oxidation activity.

Key words: Inula britannica flower, Polysaccharide, Anti-liver injury