Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus

高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (2): 249-254 .

Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus

ZHANG Na¹, LIU Yan¹, LU Jia-hui¹, WANG Juan¹, YANG Shuang¹, ZHANG Nan¹, MENG Qing-fan¹, TENG Li-rong^1,2

1. College of Life Science, Jilin University, Changchun 130012, P. R. China;
2. Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai 519041, P. R. China

收稿日期:2011-10-20 修回日期:2012-01-04 出版日期:2012-03-25 发布日期:2012-03-08
通讯作者: MENG Qing-fan, TENG Li-rong E-mail:tenglr543@gmail.com; mengqf@jlu.edu.cn
基金资助:
Supported by the Pharmaceutical Development Fund of China Medical Foundation(No.20061108).

Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus

ZHANG Na¹, LIU Yan¹, LU Jia-hui¹, WANG Juan¹, YANG Shuang¹, ZHANG Nan¹, MENG Qing-fan¹, TENG Li-rong^1,2

1. College of Life Science, Jilin University, Changchun 130012, P. R. China;
2. Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai 519041, P. R. China

Received:2011-10-20 Revised:2012-01-04 Online:2012-03-25 Published:2012-03-08
Supported by:
Supported by the Pharmaceutical Development Fund of China Medical Foundation(No.20061108).

摘要/Abstract

摘要： Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese medicine. Seven water-soluble polysaccharide fractions(ILN I, ILN II, ILN III, ILA I, ILA II, ILB I and ILB II) were isolated and purified from Irpex lacteus by hot-water extraction, deproteinization, decolorization, dicthy laminoethyl(DEAE)-cellulose ion exchange and sephadex G100 chromatographies, respectively. The average molecular weights and monosaccharide composition of these polysaccharide fractions greatly differed from each other. The antitumor and antinephritis activities of the seven polysaccharide fractions were evaluated. It was found that ILN III displayed significant inhibition effects on both humar hepatocellular liver carcinoma(HepG2) and hentietta lacks(HeLa) tumor cells with IC₅₀ values of 60.95 and 99.95 μg/mL, respectively. ILA I exhibited significant inhibition effects on murine mesangial cells(HBZT-1) with an IC50 value of 185.06 μg/mL. The inhibition effects of other polysaccharide fractions on these three cells were significantly different. These results suggest that the polysaccharide fractions isolated from Irpex lacteus have potential antitumor and antinephritis activities.

关键词: Irpex lacteus, Polysaccharide, Purification, Bioactivity

Abstract: Irpex lacteus has been widely used for treating chronic glomerulonephritis as a traditional Chinese medicine. Seven water-soluble polysaccharide fractions(ILN I, ILN II, ILN III, ILA I, ILA II, ILB I and ILB II) were isolated and purified from Irpex lacteus by hot-water extraction, deproteinization, decolorization, dicthy laminoethyl(DEAE)-cellulose ion exchange and sephadex G100 chromatographies, respectively. The average molecular weights and monosaccharide composition of these polysaccharide fractions greatly differed from each other. The antitumor and antinephritis activities of the seven polysaccharide fractions were evaluated. It was found that ILN III displayed significant inhibition effects on both humar hepatocellular liver carcinoma(HepG2) and hentietta lacks(HeLa) tumor cells with IC₅₀ values of 60.95 and 99.95 μg/mL, respectively. ILA I exhibited significant inhibition effects on murine mesangial cells(HBZT-1) with an IC50 value of 185.06 μg/mL. The inhibition effects of other polysaccharide fractions on these three cells were significantly different. These results suggest that the polysaccharide fractions isolated from Irpex lacteus have potential antitumor and antinephritis activities.

Key words: Irpex lacteus, Polysaccharide, Purification, Bioactivity

ZHANG Na, LIU Yan, LU Jia-hui, WANG Juan, YANG Shuang, ZHANG Nan, MENG Qing-fan, TENG Li-rong. Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus[J]. 高等学校化学研究, 2012, 28(2): 249-254 .

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Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus

Isolation, Purification and Bioactivities of Polysaccharides from Irpex lacteus

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