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高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (3): 474-477.

• Articles • 上一篇    下一篇

Expression and Characterization of the Intracellular Part of Receptor Protein Tyrosine Phosphatase PTPRU

WANG Shao-feng1,2, HAN Jia-yu2, FU Xue-qi1* and ZHAO Zhi-zhuang Joe1,2*   

  1. 1. Edmond H. Fischer Signal Transduction Laboratory, College of Life Sciences, Jilin University, Changchun 130012, P. R. China;
    2. Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma?73104, USA
  • 收稿日期:2010-03-08 修回日期:2010-04-30 出版日期:2011-05-25 发布日期:2011-04-29
  • 通讯作者: FU Xue-qi and ZHAO Zhi-zhuang Joe E-mail:fxq@jlu.edu.cn; joe-zhao@ouhsc.edu
  • 基金资助:

    Supported by the Grant from the Plan for Development of Science & Technology in Jilin Province, China(No.20090920),  the Boyou Fund from China Soong Ching Ling Foundation and the Grant from the National Institutes of Health, USA (No.HL76309).

Expression and Characterization of the Intracellular Part of Receptor Protein Tyrosine Phosphatase PTPRU

WANG Shao-feng1,2, HAN Jia-yu2, FU Xue-qi1* and ZHAO Zhi-zhuang Joe1,2*   

  1. 1. Edmond H. Fischer Signal Transduction Laboratory, College of Life Sciences, Jilin University, Changchun 130012, P. R. China;
    2. Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma?73104, USA
  • Received:2010-03-08 Revised:2010-04-30 Online:2011-05-25 Published:2011-04-29
  • Contact: FU Xue-qi and ZHAO Zhi-zhuang Joe E-mail:fxq@jlu.edu.cn; joe-zhao@ouhsc.edu
  • Supported by:

    Supported by the Grant from the Plan for Development of Science & Technology in Jilin Province, China(No.20090920),  the Boyou Fund from China Soong Ching Ling Foundation and the Grant from the National Institutes of Health, USA (No.HL76309).

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被引次数

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摘要： PTPRU is an MAM domain-containing receptor-like protein tyrosine phosphatase. Previous studies have demonstrated an important role of the enzyme in the maintenance of epithelial integrity and in the regulation of the Wnt/?-catenin signaling pathway. To better understand the function of PTPRU, we cloned and expressed the intracellular portion of PTPRU as a GST fusion protein in E. coli cells. We purified the protein to homogeneity and used it to immunize mice for antibody production. The resultant antibody specifically recognized PTPRU over-expressed in the cell line. Western blot analyses demonstrated the partition of truncated forms of PTPRU containing the cadherin-like domain in the Triton X-100-insoluble fraction, and immunofluorescent cell staining revealed the localization of these proteins in punctate intracellular structures. Our data suggest that the cadherin-like domain of PTPRU has a major role in determining its intracellular localization.

关键词: Tyrosine phosphatase, Cadherin, Catenin, Microdomain

Abstract: PTPRU is an MAM domain-containing receptor-like protein tyrosine phosphatase. Previous studies have demonstrated an important role of the enzyme in the maintenance of epithelial integrity and in the regulation of the Wnt/?-catenin signaling pathway. To better understand the function of PTPRU, we cloned and expressed the intracellular portion of PTPRU as a GST fusion protein in E. coli cells. We purified the protein to homogeneity and used it to immunize mice for antibody production. The resultant antibody specifically recognized PTPRU over-expressed in the cell line. Western blot analyses demonstrated the partition of truncated forms of PTPRU containing the cadherin-like domain in the Triton X-100-insoluble fraction, and immunofluorescent cell staining revealed the localization of these proteins in punctate intracellular structures. Our data suggest that the cadherin-like domain of PTPRU has a major role in determining its intracellular localization.

Key words: Tyrosine phosphatase, Cadherin, Catenin, Microdomain