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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (6): 969-973.

• Articles • 上一篇    下一篇

Inhibition of Salidroside on Salivary Gland Adenoid Cystic Carcinoma

LI Mei-hua1, ZHOU Hong-lan2*, WANG Wei1 and QIU Xin-ru3   

  1. 1. Oral Department of the Third Hospital, Jilin University, Changchun 130033, P. R. China;
    2. The First Hospital, 3. College of Pharmacy, Jilin University, Changchun 130021, P. R. China
  • 收稿日期:2009-12-21 修回日期:2010-05-14 出版日期:2010-11-25 发布日期:2010-12-01
  • 通讯作者: ZHOU Hong-lan E-mail:walkerzhouhl@163.com
  • 作者简介:ZHOU Hong-lan. E-mail: walkerzhouhl@163.com
  • 基金资助:

    Supported by the Jilin Provincial Development and Reform Commission, China(No.2007969), the Jilin Provincial Science & Technology Department, China(No.20030551-10) and the China Postdoctoral Science Foundation(No.200403699).

Inhibition of Salidroside on Salivary Gland Adenoid Cystic Carcinoma

LI Mei-hua1, ZHOU Hong-lan2*, WANG Wei1 and QIU Xin-ru3   

  1. 1. Oral Department of the Third Hospital, Jilin University, Changchun 130033, P. R. China;
    2. The First Hospital, 3. College of Pharmacy, Jilin University, Changchun 130021, P. R. China
  • Received:2009-12-21 Revised:2010-05-14 Online:2010-11-25 Published:2010-12-01
  • Contact: ZHOU Hong-lan E-mail:walkerzhouhl@163.com
  • About author:ZHOU Hong-lan. E-mail: walkerzhouhl@163.com
  • Supported by:

    Supported by the Jilin Provincial Development and Reform Commission, China(No.2007969), the Jilin Provincial Science & Technology Department, China(No.20030551-10) and the China Postdoctoral Science Foundation(No.200403699).

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摘要： To evaluate the role of salidroside on proliferation, apoptosis and invasiveness of salivary gland adenoid cystic carcinoma cells(SACC), immunocytochemical staining was employed to detect proliferating cell nuclear antigen(PCNA), caspase 3 and caspase 8 expression in SACC-2 cells. Modified Boyden chamber assay combined with laser confocal microscopy(LSCM) was used to evaluate the invasion and migration abilities of SACC-2 cells at different time point. Immunohistochemistry staining revealed that the expression of PCNA was significantly decreased(P<0.01) after salidroside treatment. In contrast, salidroside treatment led to increased caspase 3 and caspase 8 in SACC-2 cells. Cell migration depth and number of cells that penetrated Boyden chamber were also decreased by salidroside. Salidroside potently inhibits the proliferation and simultaneously induces the apoptosis of SACC-2 cells. Migration and invasion of SACC-2 cells are also inhibited. Our data throw light on potential clinical application of salidroside to the patients with SACC.

关键词: Immunohistochemistry, Salidroside, Salivary gland adenoid cystic carcinoma cell, SACC-2, Boyden chamber, Proliferating cell nuclear antigen

Abstract: To evaluate the role of salidroside on proliferation, apoptosis and invasiveness of salivary gland adenoid cystic carcinoma cells(SACC), immunocytochemical staining was employed to detect proliferating cell nuclear antigen(PCNA), caspase 3 and caspase 8 expression in SACC-2 cells. Modified Boyden chamber assay combined with laser confocal microscopy(LSCM) was used to evaluate the invasion and migration abilities of SACC-2 cells at different time point. Immunohistochemistry staining revealed that the expression of PCNA was significantly decreased(P<0.01) after salidroside treatment. In contrast, salidroside treatment led to increased caspase 3 and caspase 8 in SACC-2 cells. Cell migration depth and number of cells that penetrated Boyden chamber were also decreased by salidroside. Salidroside potently inhibits the proliferation and simultaneously induces the apoptosis of SACC-2 cells. Migration and invasion of SACC-2 cells are also inhibited. Our data throw light on potential clinical application of salidroside to the patients with SACC.

Key words: Immunohistochemistry, Salidroside, Salivary gland adenoid cystic carcinoma cell, SACC-2, Boyden chamber, Proliferating cell nuclear antigen