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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (6): 964-968.

• Articles • 上一篇    下一篇

Inhibitory Effects of Phenylacetic Acid on Proliferation of Human Pancreatic Carcinoma BXPC-3 Cells by the Regulation of Adenosine Deaminases Acting on RNA

JIANG Tao1, REN Hui2, ZHANG Guang3, ZHANG Ling4, TIAN Xiao-feng1, JIA Hui-jie4, HAN Li-ying5* and TIAN Yu6*   

  1. 1. Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. Department of General Surgery, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    3. Department of Thyroid Gland Surgery, China-Japan Union Hospital of Jilin University,Changchun 130033, P. R. China;
    4. Prostate Diseases Prevention and Treatment Research Centre and Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun 130021, P. R. China;
    5. Department of Obstetrics and Gynecology, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    6. Department of Neurosurgery,China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China
  • 收稿日期:2010-01-18 修回日期:2010-07-01 出版日期:2010-11-25 发布日期:2010-12-01
  • 通讯作者: TIAN Yu;HAN Li-ying E-mail:tianyu2801@sohu.com;zanghu@163.com
  • 作者简介:TIAN Yu and HAN Li-ying . E-mail: tianyu2801@sohu.com; zanghu@163.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.30801354 and 30970791) and the Jilin Provincial Science & Technology Department, China(No.20080154).

Inhibitory Effects of Phenylacetic Acid on Proliferation of Human Pancreatic Carcinoma BXPC-3 Cells by the Regulation of Adenosine Deaminases Acting on RNA

JIANG Tao1, REN Hui2, ZHANG Guang3, ZHANG Ling4, TIAN Xiao-feng1, JIA Hui-jie4, HAN Li-ying5* and TIAN Yu6*   

  1. 1. Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. Department of General Surgery, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    3. Department of Thyroid Gland Surgery, China-Japan Union Hospital of Jilin University,Changchun 130033, P. R. China;
    4. Prostate Diseases Prevention and Treatment Research Centre and Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun 130021, P. R. China;
    5. Department of Obstetrics and Gynecology, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    6. Department of Neurosurgery,China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China
  • Received:2010-01-18 Revised:2010-07-01 Online:2010-11-25 Published:2010-12-01
  • Contact: TIAN Yu;HAN Li-ying E-mail:tianyu2801@sohu.com;zanghu@163.com
  • About author: TIAN Yu and HAN Li-ying . E-mail: tianyu2801@sohu.com; zanghu@163.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30801354 and 30970791) and the Jilin Provincial Science & Technology Department, China(No.20080154).

PDF (PC)

摘要： The effect and mechanism of phenylacetic acid on the proliferation of pancreatic carcinoma cells    were investigated in cultured pancreatic carcinoma BXPC-3 cells by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-       diphenyltetrazolium bromide assay and flow cytometry assay. The results show that the treatment of pancreatic carcinoma cells with phenylacetic acid significantly inhibited the cell proliferation in time-dependent and dose-dependent manners. The proliferation of BXPC-3 cells was inhibited at the stage of S phase, the cells at the end stage of S phase were accumulated abundantly, and thus DNA synthesis could not be accomplished entirely. In addition, the expression of adenosine deaminases acting on RNA(ADARs) mRNA in BXPC-3 cells and pancreatic carcinoma specimen were detected by RT-PCR. Having been treated with phenylacetic acid, ADAR2 mRNA in BXPC-3 cells was significantly decreased, the differences were of statistical significance(P<0.01). Taken together, these results suggest that phenylacetic acid may likely regulate the proliferation of pancreatic carcinoma cells through the regulation of ADAR2 mRNA expression.

关键词: Pancreatic carcinoma, Phenylacetic acid, ADAR2, RNA editing

Abstract: The effect and mechanism of phenylacetic acid on the proliferation of pancreatic carcinoma cells    were investigated in cultured pancreatic carcinoma BXPC-3 cells by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-       diphenyltetrazolium bromide assay and flow cytometry assay. The results show that the treatment of pancreatic carcinoma cells with phenylacetic acid significantly inhibited the cell proliferation in time-dependent and dose-dependent manners. The proliferation of BXPC-3 cells was inhibited at the stage of S phase, the cells at the end stage of S phase were accumulated abundantly, and thus DNA synthesis could not be accomplished entirely. In addition, the expression of adenosine deaminases acting on RNA(ADARs) mRNA in BXPC-3 cells and pancreatic carcinoma specimen were detected by RT-PCR. Having been treated with phenylacetic acid, ADAR2 mRNA in BXPC-3 cells was significantly decreased, the differences were of statistical significance(P<0.01). Taken together, these results suggest that phenylacetic acid may likely regulate the proliferation of pancreatic carcinoma cells through the regulation of ADAR2 mRNA expression.

Key words: Pancreatic carcinoma, Phenylacetic acid, ADAR2, RNA editing