[an error occurred while processing this directive]

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (6): 915-921.

• Articles • 上一篇    下一篇

Separation and Bio-activities of Spirostanol Saponin from Tribulus terrestris

ZHANG Shuang1, YANG Rui-jie2, LI Hong1, YIN Zong-yuan2, ZHOU Hong-yu2,LI Xu-wen2, JIN Yong-ri2* and YANG Shi-jie1*   

  1. 1. Norman Bethune College of Medicine, Jilin University, Changchun 130021, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • 收稿日期:2010-05-28 修回日期:2010-06-17 出版日期:2010-11-25 发布日期:2010-12-01
  • 通讯作者: JIN Yong-ri;YANG Shi-jie E-mail:jinyr@jlu.edu.cn; jcyaoli@sina.com
  • 作者简介:JIN Yong-ri and YANG Shi-jie. E-mail: jinyr@jlu.edu.cn; jcyaoli@sina.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(Nos.30472020 and 30672654).

Separation and Bio-activities of Spirostanol Saponin from Tribulus terrestris

ZHANG Shuang1, YANG Rui-jie2, LI Hong1, YIN Zong-yuan2, ZHOU Hong-yu2,LI Xu-wen2, JIN Yong-ri2* and YANG Shi-jie1*   

  1. 1. Norman Bethune College of Medicine, Jilin University, Changchun 130021, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • Received:2010-05-28 Revised:2010-06-17 Online:2010-11-25 Published:2010-12-01
  • Contact: JIN Yong-ri;YANG Shi-jie E-mail:jinyr@jlu.edu.cn; jcyaoli@sina.com
  • About author:JIN Yong-ri and YANG Shi-jie. E-mail: jinyr@jlu.edu.cn; jcyaoli@sina.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.30472020 and 30672654).

PDF (PC)

摘要： Gross saponins of Tribulus terrestris(GSTT) have exact effect on cardiovascular and cerebrovascular  diseases. But as a mixture, the specific efficient component of GSTT is still unknown. Nine monomers of spirostanol saponins were isolated and idendified as JA―JI(named transitorily) by means of NMR spectrometry. After bio-    activity screening on them, we defined that monomers tigogenin 3-O-β-D-xylopyranosyl(1→2)-[β-D-xylopyranosyl (1→3)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(compound JB) and hecogenin3-O-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(compound JG) have cytoprotective bio-activity. Compound JB display effective dose in 10–8 and 10–9 mol/L, and JG in 10–6―10–9 mol/L. Survival rate, lactate dehydrogenase (LDH) and apoptosis also show that JB(at dose 10–8, 10–9 and 10–10 mol/L) can protect myocardial injury caused by hypoxia/reoxygenation(H/R). While morphology change also shows JG has cytoprotective bio-activity.

关键词: Tribulus terrestris, Spirostanol monomer, Bio-activity, Cytoprotective

Abstract: Gross saponins of Tribulus terrestris(GSTT) have exact effect on cardiovascular and cerebrovascular  diseases. But as a mixture, the specific efficient component of GSTT is still unknown. Nine monomers of spirostanol saponins were isolated and idendified as JA―JI(named transitorily) by means of NMR spectrometry. After bio-    activity screening on them, we defined that monomers tigogenin 3-O-β-D-xylopyranosyl(1→2)-[β-D-xylopyranosyl (1→3)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(compound JB) and hecogenin3-O-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(compound JG) have cytoprotective bio-activity. Compound JB display effective dose in 10–8 and 10–9 mol/L, and JG in 10–6―10–9 mol/L. Survival rate, lactate dehydrogenase (LDH) and apoptosis also show that JB(at dose 10–8, 10–9 and 10–10 mol/L) can protect myocardial injury caused by hypoxia/reoxygenation(H/R). While morphology change also shows JG has cytoprotective bio-activity.

Key words: Tribulus terrestris, Spirostanol monomer, Bio-activity, Cytoprotective