Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (5): 798-802.

Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris

ZHANG Ai-li, LU Jia-hui, ZHANG Na, ZHENG Dan, ZHANG Gui-rong* and TENG Li-rong*

College of Life Science, Jilin University, Changchun 130012, P. R. China

收稿日期:2010-03-10 修回日期:2010-03-17 出版日期:2010-09-25 发布日期:2010-11-26
通讯作者: TENG Li-rong. E-mail: tenglr@jlu.edu.cn; ZHANG Gui-rong. E-mail: zgr@jlu.edu.cn
基金资助:
Supported by the Xiuzheng Pharmaceutical Group Ltd. Commercial Project, China(No.2007220101002580).

Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris

ZHANG Ai-li, LU Jia-hui, ZHANG Na, ZHENG Dan, ZHANG Gui-rong* and TENG Li-rong*

College of Life Science, Jilin University, Changchun 130012, P. R. China

Received:2010-03-10 Revised:2010-03-17 Online:2010-09-25 Published:2010-11-26
Contact: TENG Li-rong. E-mail: tenglr@jlu.edu.cn; ZHANG Gui-rong. E-mail: zgr@jlu.edu.cn
Supported by:
Supported by the Xiuzheng Pharmaceutical Group Ltd. Commercial Project, China(No.2007220101002580).

摘要/Abstract

摘要： A water-soluble polysaccharide(named MCMP) was isolated from the mycelium with high yield mutation Cordyceps militaris by hot-water extraction, deproteinization by sevage, alcohol precipitation, anion-exchange and gel filtration chromatography CL-6B. The polysaccharide contained mannose, rhamnose, galactose and glucose in a molar ratio of 59.36:1:8.31:39.50, of which the average molecular weight is 8100. In our research, Hep-G2 cells, Hela cells and mesangial cells were chosen to determine the anti-tumor activity of the polysaccharide. The results of MTT assay show that polysaccharides of the mutant strain presented inhibitory activity on the cells proliferation after 48 h incubation.

关键词: Cordyceps militaris, Polysaccharide, Anti-tumor

Abstract: A water-soluble polysaccharide(named MCMP) was isolated from the mycelium with high yield mutation Cordyceps militaris by hot-water extraction, deproteinization by sevage, alcohol precipitation, anion-exchange and gel filtration chromatography CL-6B. The polysaccharide contained mannose, rhamnose, galactose and glucose in a molar ratio of 59.36:1:8.31:39.50, of which the average molecular weight is 8100. In our research, Hep-G2 cells, Hela cells and mesangial cells were chosen to determine the anti-tumor activity of the polysaccharide. The results of MTT assay show that polysaccharides of the mutant strain presented inhibitory activity on the cells proliferation after 48 h incubation.

Key words: Cordyceps militaris, Polysaccharide, Anti-tumor

ZHANG Ai-li, LU Jia-hui, ZHANG Na, ZHENG Dan, ZHANG Gui-rong* and TENG Li-rong*. Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris[J]. 高等学校化学研究, 2010, 26(5): 798-802.

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Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris

Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris

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