Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (2): 323-330.

Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

JIN Gu* and YAO Jian-xia

Department of Chemistry, University of Science and Technology of China, Hefei 230026, P. R. China

收稿日期:2008-12-05 修回日期:2009-08-04 出版日期:2010-03-25 发布日期:2010-05-25
通讯作者: JIN Gu. E-mail: gjin@ustc.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.29405038).

Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

JIN Gu* and YAO Jian-xia

Department of Chemistry, University of Science and Technology of China, Hefei 230026, P. R. China

Received:2008-12-05 Revised:2009-08-04 Online:2010-03-25 Published:2010-05-25
Contact: JIN Gu. E-mail: gjin@ustc.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.29405038).

摘要/Abstract

摘要：

Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethylene(MPTFE) capillary. The new adsorbent comprised grafted glycidyl methacrylate(GMA) via radiation-induced polymerization as hydrophilic coating and reactive sites; ethylenediamine(EDA) as a spacer arm; Cibacron Blue F3GA(CB F3GA) as an affinity ligand; MPTFE capillary as the supporting matrix. The average density of CB F3GA attachment to MPTFE capillaries was found to be 136.5 μmol/g. The capacity of bilirubin adsorbed on affinity MPTFE capillaries is 76.1 mg bilirubin/g polymer(at 25 °C). This new adsorbent has advantages over both membrane and traditional micro-column, and this system is stable and easy to operate. The results of blood tests suggest the CB F3GA affinity capillary has good blood compatibility.

关键词: Bilirubin removal; Radiation grafting; Cibacron Blue F3GA; Polytetrafluoroethylene; Microporous capillary

Abstract:

Key words: Bilirubin removal; Radiation grafting; Cibacron Blue F3GA; Polytetrafluoroethylene; Microporous capillary

JIN Gu* and YAO Jian-xia. Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary[J]. 高等学校化学研究, 2010, 26(2): 323-330.

JIN Gu* and YAO Jian-xia. Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary[J]. Chemical Research in Chinese Universities, 2010, 26(2): 323-330.

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Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

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