高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (1): 23-26.
Application of Colloidal Gold to Fluorophotometric Determination of Human IgG
DENG Xin-yu1, GAO De-jiang1, TIAN Yuan1, CHEN Yan-hua1, YU Ai-min1, ZHANG Han-qi1, WANG Xing-hua1* and CHEN Ying2
- 1. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
2. Jilin Province Center of Environmental Monitoring, Changchun 130011, P. R. China
Application of Colloidal Gold to Fluorophotometric Determination of Human IgG
DENG Xin-yu1, GAO De-jiang1, TIAN Yuan1, CHEN Yan-hua1, YU Ai-min1, ZHANG Han-qi1, WANG Xing-hua1* and CHEN Ying2
- 1. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
2. Jilin Province Center of Environmental Monitoring, Changchun 130011, P. R. China
摘要：
A method for the determination of human immunoglobulin G(IgG) based on a colloidal gold label by fluorospectrophotometry was developed. The sandwich immunoreaction among goat-anti-human IgG, human IgG and goat-anti-human IgG labeled with colloidal gold nanoparticles was applied in this experiment. First, a sandwich immunocomplex was formed on the surface of 96 well clear polystyrene high bind stripwellTM microplate. After the formation of the sandwich immunocomplex, a solution was added to dissociate the immunocomplex at room tempe- rature. Then the solution of each well was transferred into the corresponding test tube. Thirdly, the rhodamine B solution was injected into each test tube. The rhodamine B chloraurate was extracted into ether that was measured with a spectrofluorometer. The experimental results indicate that the fluorescence intensity increased with the increase of human IgG concentration. The fluorescence intensity of rhodamine B chloraurate at 570 nm was proportional to the logarithm of human IgG concentration in a range from 10 to 5×105 ng/mL. It was shown that the determination of human IgG was easily made with the proposed fluorospectrophotometry.